extractSwitchOverlap: Visualize Switch Overlap

In IsoformSwitchAnalyzeR: Identify, Annotate and Visualize Alternative Splicing and Isoform Switches with Functional Consequences from both short- and long-read RNA-seq data.

Description

This function produces two Venn diagrams respectively showing the overlap in switching isoforms and genes.

Usage

extractSwitchOverlap(
    switchAnalyzeRlist,
    filterForConsequences = FALSE,
    alpha = 0.05,
    dIFcutoff = 0.1,
    scaleVennIfPossible=TRUE,
    plotIsoforms = TRUE,
    plotSwitches = TRUE,
    plotGenes = TRUE
)

Arguments

switchAnalyzeRlist	A switchAnalyzeRlist object.
filterForConsequences	A logical indicating whether to filter for genes with functional consequences. Requires that analyzeSwitchConsequences() have been run on the switchAnalyzeRlist. The output will then be the number of significant genes and isoforms originating from genes with predicted consequences. Default is FALSE.
alpha	The cutoff which the FDR correct p-values must be smaller than for calling significant switches. Default is 0.05.
dIFcutoff	The cutoff which the changes in (absolute) isoform usage must be larger than before an isoform is considered switching. This cutoff can remove cases where isoforms with (very) low dIF values are deemed significant and thereby included in the downstream analysis. This cutoff is analogous to having a cutoff on log2 fold change in a normal differential expression analysis of genes to ensure the genes have a certain effect size. Default is 0.1 (10%).
scaleVennIfPossible	A logic indicating whether the Venn diagram should be scaled (so the circle area and overlap size reflect the number of features) if possible. Only available for 2- and 3-way Venn Diagrams. Default is TRUE.
plotIsoforms	A logic indicating whether the Venn diagram of differentially used isoforms should be plotted. Default is TRUE.
plotSwitches	A logic indicating whether the Venn diagram of identified isoform switches should be plotted. Default is TRUE.
plotGenes	A logic indicating whether the Venn diagram of genes containing differentially used isoforms should be plotted. Default is TRUE.

Value

A Venn diagram which shows the number of isoforms and genes with a isoform switch.

Author(s)

Kristoffer Vitting-Seerup

References

• Vitting-Seerup et al. The Landscape of Isoform Switches in Human Cancers. Mol. Cancer Res. (2017).

See Also

preFilter
isoformSwitchTestDEXSeq
isoformSwitchTestDRIMSeq
extractTopSwitches
extractSwitchSummary
analyzeSwitchConsequences

Examples

# Load example data and prefilter
data("exampleSwitchListAnalyzed")

extractSwitchOverlap(exampleSwitchListAnalyzed)

IsoformSwitchAnalyzeR documentation built on Nov. 8, 2020, 5:36 p.m.