calcMW: Calculate molecular weight

In MassArray: Analytical Tools for MassArray Data

Description

Function to calculate molecular weight of a fragment generated by the MassCLEAVE assay for either the T or C cleavage reactions

Usage

calcMW(sequence, extra = c("5OH", "5PPP-3P", "5PPP-3OH"), adduct = c("", "Na", "K"), rxn = c("T", "C"))

Arguments

sequence	Nucleotide sequence input
extra	One of "5OH" (default), "5PPP-3P", or "5PPP-3OH" describing 5' and/or 3' modifications of the fragment
adduct	One of 'Na', 'K', or ” (default) specifying whether or not the molecular weight should be calculated for a salt adduct
rxn	One of 'T' or 'C' indicating which cleavage reaction is employed to generate the fragment

Value

Returns a numerical output corresponding to the molecular weight (in Da) of sequence input. Note that the output may actually represent multiple molecular weights if/whenever the input sequence contains one or more degenerate bases (e.g. R or Y).

Author(s)

Reid F. Thompson ([e-mail]), John M. Greally ([e-mail])

Examples

calcMW("AAATCCC")
calcMW("AARTYCC")

Example output

[1] 2180.377
[1] 2197.361 2180.377 2213.360 2196.376

MassArray documentation built on Nov. 8, 2020, 5:16 p.m.