This data set contains two-color miRNA microarray expression data obtained from mouse embryonic tissue during gestational days (GD) 12, 13, and 14, which represents the critical period of palate development in the mouse.
The data are in the format of an
which in this case is a list with the following 9 elements:
matrix of dimension 6336 x 9 which contains the red channel foreground measurements
matrix of dimension 6336 x 9 which contains the green channel foreground measurements
matrix of dimension 6336 x 9 which contains the red channel background measurements
matrix of dimension 6336 x 9 which contains the green channel background measurements
source of the images, here "imagene"
numeric vector giving the field dimensions of the array (Metarows, Metacols, Rows and Cols)
matrix of dimension 6336 x 9 which contains the quality weights associated with each spot on the arrays
list containing information on the process
used to print the spots on the arrays (number of grid rows /
columns and number of spot rows / columns per grid - coincides
data.frame containing information on
each probe. Has the following columns:
field position for the probe
meta row position for the probe
meta column position for the probe
row position for the probe
column position for the probe
unique gene identifier provided by Miltenyi Biotec
unique probe identifier constructed by concatentating the "Gene ID" with "Meta Row", "Meta Column", "Row", and "Column" information
name of the microRNA
base name of the microRNA
type of probe, "MMU miRNAs", "Other miRNAs", "Control", "Empty", and "Other"
RNA samples were isolated from mouse embryonic orofacial tissues (GD-12 - GD-14) and fluorescently labeled with Hy5 (red). Control samples (miRXplore Universal Reference) were labeled with Hy3 (green). The two sets of samples were hybridized to miRXplore Microarrays (Miltenyi Biotec) sing the a-Hyb Hybridization Station (Miltenyi Biotec). Probes for a total of 1336 mature miRNAs (from human, mouse, rat and virus), including positive control and calibration probes, were spotted in quadruplicate on each microarray. Each array included probes for 588 murine miRNAs.
P. Mukhopadhyay, G. Brock, V. Pihur, C. Webb, M.M. Pisano, and R.M. Greene. Developmental microRNA expression profiling of murine embryonic orofacial tissue. Birth Defects Res A Clin Mol Teratol, 88(7):511-34, 2010.