siteAnnotate: Phosphosite annotation

In PhosR: A set of methods and tools for comprehensive analysis of phosphoproteomics data

Description

This function plots the combined scores of each of all kinases for a given phosphosites

Usage

siteAnnotate(site, phosScoringMatrices, predMatrix)

Arguments

site	site the ID of a phosphosite
phosScoringMatrices	output from function kinaseSubstrateScore()
predMatrix	a prediction matrix from kinaseSubstratePred()

Value

A graphical plot

Examples

data('phospho_L6_ratio')
data('SPSs')

grps = gsub('_.+', '', colnames(phospho.L6.ratio))

# Cleaning phosphosite label
phospho.site.names = rownames(phospho.L6.ratio)
L6.sites = gsub(' ', '', sapply(strsplit(rownames(phospho.L6.ratio), '~'),
                                function(x){paste(toupper(x[2]), x[3], '',
                                                sep=';')}))
phospho.L6.ratio = t(sapply(split(data.frame(phospho.L6.ratio), L6.sites),
                            colMeans))
phospho.site.names = split(phospho.site.names, L6.sites)

# Construct a design matrix by condition
design = model.matrix(~ grps - 1)

# phosphoproteomics data normalisation using RUV
ctl = which(rownames(phospho.L6.ratio) %in% SPSs)
phospho.L6.ratio.RUV = RUVphospho(phospho.L6.ratio, M = design, k = 3,
                                ctl = ctl)


phosphoL6 = phospho.L6.ratio.RUV
rownames(phosphoL6) = phospho.site.names

# filter for up-regulated phosphosites
phosphoL6.mean <- meanAbundance(phosphoL6,
                                grps = gsub('_.+', '', colnames(phosphoL6)))
aov <- matANOVA(mat=phosphoL6, grps=gsub('_.+', '', colnames(phosphoL6)))
phosphoL6.reg <- phosphoL6[(aov < 0.05) &
                        (rowSums(phosphoL6.mean > 0.5) > 0),,drop = FALSE]
L6.phos.std <- standardise(phosphoL6.reg)
rownames(L6.phos.std) <- sapply(strsplit(rownames(L6.phos.std), '~'),
    function(x){gsub(' ', '', paste(toupper(x[2]), x[3], '', sep=';'))})

L6.phos.seq <- sapply(strsplit(rownames(phosphoL6.reg), '~'),
                    function(x)x[4])

L6.matrices <- kinaseSubstrateScore(PhosphoSite.mouse, L6.phos.std,
    L6.phos.seq, numMotif = 5, numSub = 1)
set.seed(1)
L6.predMat <- kinaseSubstratePred(L6.matrices, top=30)

# We will look at the phosphosite AAK1;S677 for demonstration purpose.
site = "AAK1;S677;"
siteAnnotate(site, L6.matrices, L6.predMat)

PhosR documentation built on Nov. 8, 2020, 6:54 p.m.