Description Available methods Author(s) See Also Examples
These are methods for getting or setting assay(sce, i=X, ...)
where sce
is a SingleCellExperiment object and X
is the name of the method.
For example, counts
will get or set X="counts"
.
This provides some convenience for users as well as encouraging standardization of assay names across packages.
In the following code snippets, x
is a SingleCellExperiment object,
value
is a matrix-like object with the same dimensions as x
,
and ...
are further arguments passed to assay
(for the getter) or assay<-
(for the setter).
counts(x, ...)
, counts(x, ...) <- value
:Get or set a matrix of raw count data, e.g., number of reads or transcripts.
normcounts(x, ...)
, normcounts(x, ...) <- value
:Get or set a matrix of normalized values on the same scale as the original counts. For example, counts divided by cell-specific size factors that are centred at unity.
logcounts(x, ...)
, logcounts(x, ...) <- value
:Get or set a matrix of log-transformed counts or count-like values.
In most cases, this will be defined as log-transformed normcounts
, e.g., using log base 2 and a pseudo-count of 1.
cpm(x, ...)
, cpm(x, ...) <- value
:Get or set a matrix of counts-per-million values. This is the read count for each gene in each cell, divided by the library size of each cell in millions.
tpm(x, ...)
, tpm(x, ...) <- value
:Get or set a matrix of transcripts-per-million values. This is the number of transcripts for each gene in each cell, divided by the total number of transcripts in that cell (in millions).
weights(x, ...)
, weights(x, ...) <- value
:Get or set a matrix of weights, e.g., observational weights to be used in differential expression analysis.
Aaron Lun
assay
and assay<-
, for the wrapped methods.
1 2 3 4 5 6 7 8 9 10 11 12 13 | example(SingleCellExperiment, echo=FALSE) # Using the class example
counts(sce) <- matrix(rnorm(nrow(sce)*ncol(sce)), ncol=ncol(sce))
dim(counts(sce))
# One possible way of computing normalized "counts"
sf <- 2^rnorm(ncol(sce))
sf <- sf/mean(sf)
normcounts(sce) <- t(t(counts(sce))/sf)
dim(normcounts(sce))
# One possible way of computing log-counts
logcounts(sce) <- log2(normcounts(sce)+1)
dim(normcounts(sce))
|
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