decontX: Contamination estimation with decontX

In celda: CEllular Latent Dirichlet Allocation

Description

Identifies contamination from factors such as ambient RNA in single cell genomic datasets.

Usage

decontX(x, ...)

## S4 method for signature 'SingleCellExperiment'
decontX(
  x,
  assayName = "counts",
  z = NULL,
  batch = NULL,
  maxIter = 500,
  delta = c(10, 10),
  estimateDelta = TRUE,
  convergence = 0.001,
  iterLogLik = 10,
  varGenes = 5000,
  dbscanEps = 1,
  seed = 12345,
  logfile = NULL,
  verbose = TRUE
)

## S4 method for signature 'ANY'
decontX(
  x,
  z = NULL,
  batch = NULL,
  maxIter = 500,
  delta = c(10, 10),
  estimateDelta = TRUE,
  convergence = 0.001,
  iterLogLik = 10,
  varGenes = 5000,
  dbscanEps = 1,
  seed = 12345,
  logfile = NULL,
  verbose = TRUE
)

Arguments

x	A numeric matrix of counts or a SingleCellExperiment with the matrix located in the assay slot under assayName. Cells in each batch will be subsetted and converted to a sparse matrix of class dgCMatrix from package Matrix before analysis. This object should only contain filtered cells after cell calling. Empty cell barcodes (low expression droplets before cell calling) are not needed to run DecontX.
...	For the generic, further arguments to pass to each method.
assayName	Character. Name of the assay to use if x is a SingleCellExperiment.
z	Numeric or character vector. Cell cluster labels. If NULL, PCA will be used to reduce the dimensionality of the dataset initially, 'umap' from the 'uwot' package will be used to further reduce the dataset to 2 dimenions and the 'dbscan' function from the 'dbscan' package will be used to identify clusters of broad cell types. Default NULL.
batch	Numeric or character vector. Batch labels for cells. If batch labels are supplied, DecontX is run on cells from each batch separately. Cells run in different channels or assays should be considered different batches. Default NULL.
maxIter	Integer. Maximum iterations of the EM algorithm. Default 500.
delta	Numeric Vector of length 2. Concentration parameters for the Dirichlet prior for the contamination in each cell. The first element is the prior for the native counts while the second element is the prior for the contamination counts. These essentially act as pseudocounts for the native and contamination in each cell. If estimateDelta = TRUE, this is only used to produce a random sample of proportions for an initial value of contamination in each cell. Then fit_dirichlet is used to update delta in each iteration. If estimateDelta = FALSE, then delta is fixed with these values for the entire inference procedure. Fixing delta and setting a high number in the second element will force decontX to be more aggressive and estimate higher levels of contamination at the expense of potentially removing native expression. Default c(10, 10).
estimateDelta	Boolean. Whether to update delta at each iteration.
convergence	Numeric. The EM algorithm will be stopped if the maximum difference in the contamination estimates between the previous and current iterations is less than this. Default 0.001.
iterLogLik	Integer. Calculate log likelihood every iterLogLik iteration. Default 10.
varGenes	Integer. The number of variable genes to use in dimensionality reduction before clustering. Variability is calcualted using modelGeneVar function from the 'scran' package. Used only when z is not provided. Default 5000.
dbscanEps	Numeric. The clustering resolution parameter used in 'dbscan' to estimate broad cell clusters. Used only when z is not provided. Default 1.
seed	Integer. Passed to with_seed. For reproducibility, a default value of 12345 is used. If NULL, no calls to with_seed are made.
logfile	Character. Messages will be redirected to a file named 'logfile'. If NULL, messages will be printed to stdout. Default NULL.
verbose	Logical. Whether to print log messages. Default TRUE.

Value

If x is a matrix-like object, a list will be returned with the following items:

decontXcounts:

The decontaminated matrix. Values obtained from the variational inference procedure may be non-integer. However, integer counts can be obtained by rounding, e.g. round(decontXcounts).

contamination:

Percentage of contamination in each cell.

estimates:

List of estimated parameters for each batch. If z was not supplied, then the UMAP coordinates used to generated cell cluster labels will also be stored here.

z:

Cell population/cluster labels used for analysis.

runParams:

List of arguments used in the function call.

If x is a SingleCellExperiment, then the decontaminated counts will be stored as an assay and can be accessed with decontXcounts(x). The contamination values and cluster labels will be stored in colData(x). estimates and runParams will be stored in metadata(x)$decontX. The UMAPs used to generated cell cluster labels will be stored in reducedDims slot in x.

Examples

# Generate matrix with contamination
s <- simulateContamination(seed = 12345)

library(SingleCellExperiment)
sce <- SingleCellExperiment(list(counts = s$observedCounts))
sce <- decontX(sce)

# Plot contamination on UMAP
plotDecontXContamination(sce)

# Plot decontX cluster labels
umap <- reducedDim(sce)
plotDimReduceCluster(x = sce$decontX_clusters,
    dim1 = umap[, 1], dim2 = umap[, 2], )

# Plot percentage of marker genes detected
# in each cell cluster before decontamination
s$markers
plotDecontXMarkerPercentage(sce, markers = s$markers, assayName = "counts")

# Plot percentage of marker genes detected
# in each cell cluster after contamination
plotDecontXMarkerPercentage(sce, markers = s$markers,
                            assayName = "decontXcounts")

# Plot percentage of marker genes detected in each cell
# comparing original and decontaminated counts side-by-side
plotDecontXMarkerPercentage(sce, markers = s$markers,
                            assayName = c("counts", "decontXcounts"))

# Plot raw counts of indiviual markers genes before
# and after decontamination
plotDecontXMarkerExpression(sce, unlist(s$markers))

celda documentation built on Nov. 8, 2020, 8:24 p.m.