polygonChrom: Functions to plot circular chromosomes informations

In ecolitk: Meta-data and tools for E. coli

Description

Functions to plot circular chromosomes informations

Usage

cPlotCircle(radius=1, xlim=c(-2, 2), ylim=xlim, edges=300, main=NULL,
            main.inside, ...)

chromPos2angle(pos, len.chrom, rot=pi/2, clockwise=TRUE)

polygonChrom(begin, end, len.chrom, radius.in, radius.out,
             total.edges = 300,
             edges = max(round(abs(end - begin)/len.chrom *
                     total.edges), 2, na.rm = TRUE),
             rot = pi/2, clockwise = TRUE, ...)

linesChrom(begin, end, len.chrom, radius,
             total.edges = 300,
             edges = max(round(abs(end - begin)/len.chrom *
                     total.edges), 2, na.rm = TRUE),
             rot = pi/2, clockwise = TRUE, ...)

ecoli.len

Arguments

radius	radius
xlim, ylim	range for the plot. Can be used to zoom-in a particular region.
pos	position (nucleic base coordinate)
begin	begining of the segment (nucleic base number).
end	end of the segment (nucleic base number).
len.chrom	length of the chromosome in base pairs
radius.in	inner radius
radius.out	outer radius
total.edges	total number of edges for the chromosome
edges	number of edges for the specific segment(s)
rot	rotation (default is pi / 2, bringing the angle zero at 12 o'clock)
clockwise	rotate clockwise. Default to TRUE.
main, main.inside	main titles for the plot
...	optional graphical parameters

Details

The function chromPos2angle is a convenience function. The variable ecoli.len contains the size of the Escheria coli genome considered (K12).

Value

Except chromPos2angle, the function are solely used for their border effects.

Author(s)

laurent <[e-mail]>

Examples

data(ecoligenomeSYMBOL2AFFY)
data(ecoligenomeCHRLOC)

## find the operon lactose ("lac*" genes)
lac.i <- grep("^lac", ls(ecoligenomeSYMBOL2AFFY))
lac.symbol <- ls(ecoligenomeSYMBOL2AFFY)[lac.i]
lac.affy <- unlist(lapply(lac.symbol, get, envir=ecoligenomeSYMBOL2AFFY))

beg.end <- lapply(lac.affy, get, envir=ecoligenomeCHRLOC)
beg.end <- matrix(unlist(beg.end), nc=2, byrow=TRUE)

lac.o <- order(beg.end[, 1])

lac.i <- lac.i[lac.o]
lac.symbol <- lac.symbol[lac.o]
lac.affy <- lac.affy[lac.o]
beg.end <- beg.end[lac.o, ]

lac.col <- rainbow(length(lac.affy))

par(mfrow=c(2,2))

## plot

cPlotCircle(main="lac genes")
polygonChrom(beg.end[, 1], beg.end[, 2], ecoli.len, 1, 1.2, col=lac.col)
rect(0, 0, 1.1, 1.1, border="red")

cPlotCircle(xlim=c(0, 1.2), ylim=c(0, 1.1))
polygonChrom(beg.end[, 1], beg.end[, 2], ecoli.len, 1, 1.1, col=lac.col)
rect(0.4, 0.8, 0.7, 1.1, border="red")

cPlotCircle(xlim=c(.45, .5), ylim=c(.85, 1.0))
polygonChrom(beg.end[, 1], beg.end[, 2], ecoli.len, 1, 1.03, col=lac.col)

mid.genes <- apply(beg.end, 1, mean)
mid.angles <- chromPos2angle(mid.genes, ecoli.len)
xy <- polar2xy(1.03, mid.angles)
xy.labels <- data.frame(x = seq(0.45, 0.5, length=4), y = seq(0.95, 1.0, length=4))
segments(xy$x, xy$y, xy.labels$x, xy.labels$y, col=lac.col)
text(xy.labels$x, xy.labels$y, lac.symbol, col=lac.col)

ecolitk documentation built on Nov. 8, 2020, 5:29 p.m.