Description Creating Object Slots Accessors Methods Author(s) References See Also Examples
An object of class "Template
" summarizes a group of samples belonging to same biological-class with a class-template. A template is represented by a collection of meta-clusters (MetaCluster
) created from samples of same class. An object of class "Template
" therefore stores a list of MetaCluster
objects and other necessary parameters.
An object of class "Template
" can be created using the function create.template
:
create.template(clustSamples, dist.type = "Mahalanobis", unmatch.penalty=999999, template.id = NA_integer_)
.
The arguments to the create.template
function is described below:
clustSamples:
A list of ClusteredSample
objects from which the template is created. The working examples describe how this objects are created by clustering FC samples.
dist.type:
character, indicating the method with which the dissimilarity between a pair of clusters is computed. Supported dissimilarity measures are: 'Mahalanobis', 'KL' and 'Euclidean'. If this argument is not passed then 'Mahalanobis' distance is used by default.
unmatch.penalty:
A numeric value denoting the penalty for leaving a cluster unmatched. This parameter should be already known or be estimated empirically estimated from data (see the reference for a discussion). Default is set to a very high value so that no cluster remains unmatched.
template.id:
integer, denoting the index of the template (relative to other template). Default is NA_integer_
num.metaclusters
:The number of meta-clusters in the template.
metaClusters
:A list of length num.metaclusters
storing the meta-clusters. Each meta-cluster is stored as an object of class MetaCluster
.
dimension
:Dimensionality of the samples from which the template is created.
size
:Number of cells in the template (summation of all meta-cluster sizes).
tree
: A list (similar to an hclust
object) storing the hierarchy of the samples in a template.
template.id
:integer, denoting the index of the template (relative to other templates). Default is NA_integer_
All the slot accessor functions take an object of class Template
. I show usage of the first accessor function. Other functions can be called similarly.
get.size
:Number of cells in the template (summation of all meta-cluster sizes).
Usage: get.size(object)
here object
is a Template
object.
get.num.metaclusters
:Returns the number of meta-clusters in the template.
get.metaClusters
:Returns a list of length num.metaclusters
storing the meta-clusters. Each meta-cluster is stored as an object of class MetaCluster
.
get.dimension
:Returns the dimensionality of the samples from which the template is created.
get.tree
:Returns a hclust
object storing the hierarchy of the samples in a template.
get.template.id
:Returns the index of the template (relative to other templates).
Display details about the Template
object.
Return descriptive summary for each MetaCluster
of a Template.
Usage: summary(Template)
We plot a template as a collection of bivariate contour plots of its meta-clusters.
To plot each meta-cluster we consider the clusters within the meta-cluster normally distributed and represent each cluster with an ellipsoid. The axes of an ellipsoid is estimated from the eigen values and eigen vectors of the covariance matrix of a cluster ("Applied Multivariate Statistical Analysis" by R. Johnson and D. Wichern, 5th edition, Prentice hall). We then plot the bivariate projection of the ellipsoid as 2-D ellipses.
Usage:
plot(template, alpha=.05, plot.mc=FALSE, color.mc=NULL, colorbysample=FALSE, ...)
the arguments of the plot function are:
template:
An object of class Template
for which the plot function is invoked.
alpha:
(1-alpha)*100% quantile of the distribution of the clusters or meta-cluster is plotted.
plot.mc:
TRUE/FALSE, when TRUE the functions draws contour of the combined meta-cluster and when FALSE the function draws the contours of the individual clusters.
color.mc:
A character vector of length num.metaclusters
denoting the colors to be used to draw the contours. The ith color of this vector is used to draw the ellipses denoting clusters in the ith meta-cluster or the combined ith meta-cluster (depending on the argument plot.mc
). By default an empty vector is passed and then an arbitrary color is used to draw each meta-cluster.
colorbysample:
TRUE/FALSE, when TRUE the functions draws clusters from same samples in a single color and when FALSE the function draws meta-clusters in a single color.
... :
Other usual plotting related parameters.
Plot the hierarchy of samples established while creating the template-tree. See template.tree
Ariful Azad
Azad, Ariful and Pyne, Saumyadipta and Pothen, Alex (2012), Matching phosphorylation response patterns of antigen-receptor-stimulated T cells via flow cytometry; BMC Bioinformatics, 13 (Suppl 2), S10.
MetaCluster
, ClusteredSample
, create.template
, template.tree
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 | ## ------------------------------------------------
## load data
## ------------------------------------------------
library(healthyFlowData)
data(hd)
## ------------------------------------------------
## Retrieve each sample, clsuter it and store the
## clustered samples in a list
## ------------------------------------------------
set.seed(1234) # for reproducable clustering
cat('Clustering samples: ')
clustSamples = list()
for(i in 1:length(hd.flowSet))
{
cat(i, ' ')
sample1 = exprs(hd.flowSet[[i]])
clust1 = kmeans(sample1, centers=4, nstart=20)
cluster.labels1 = clust1$cluster
clustSample1 = ClusteredSample(labels=cluster.labels1, sample=sample1)
clustSamples = c(clustSamples, clustSample1)
}
## ------------------------------------------------
## Create a template from the list of clustered samples and plot functions
## ------------------------------------------------
template = create.template(clustSamples)
summary(template)
## plot the tree denoting the hierarchy of the samples in a template
tree = template.tree(template)
## plot the template in terms of the meta-clusters
## option-1 (default): plot contours of each cluster of the meta-clusters
plot(template)
## option-2: plot contours of each cluster of the meta-clusters with defined color
plot(template, color.mc=c('blue','black','green3','red'))
## option-3: plot contours of the meta-clusters with defined color
plot(template, plot.mc=TRUE, color.mc=c('blue','black','green3','red'))
## option-4: plot contours of each cluster of the meta-clusters with different colors for different samples
plot(template, colorbysample=TRUE)
|
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.