tidyFlow: Generate a tidy dataset from time-course flow cytometry data

Description Usage Arguments Value Examples

View source: R/noSummaryAnalysis.R

Description

Generates a tibble containing all parameters and phenoData from a flowSet which can be used to visualize and analyze timecourse flow cytometry data.

Usage

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tidyFlow(flowset, gated = FALSE, ploidy = NA, only = NA)

Arguments

flowset

your flowSet to be analyzed

gated

boolean is the data already gated?

ploidy

character gate to subset your flowset based on the ploidy of you strains

only

character which population of events to analyze, 'yeast', singlets', or 'doublets'?

Value

a data frame containing all of the selected subset of events from the original flowSet for all parameters including experiment time, etime, the time after the initial reading at which each event was collected.

Examples

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plate1<-read.flowSet(path=system.file("extdata", "tc_example",
package = "flowTime"), alter.names = TRUE)
annotation <- read.csv(system.file("extdata", "tc_example.csv",
package = "flowTime"))
plate1 <- annotateFlowSet(plate1, annotation)
tidy_dat <- tidyFlow(plate1, gated = TRUE)
head(tidy_dat)

flowTime documentation built on Nov. 8, 2020, 8:13 p.m.