global.norm: Global Normalization of Spot

Description Usage Arguments Value Examples

View source: R/operations.R

Description

This function normalize R and I values and fit the value of Cy5 from his argument. In this function the normalize algorithm will be applied to all observations to get the lowess factor and then fit Cy5 with this factor. The observations. The observations with values R = 0 are deleted because they have no change in their expression levels.

Usage

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global.norm(mySpot)

Arguments

mySpot

A spot object

Value

A new spot object but normalized, It means with a different Cy5 that is the result of the fit with the lowess factor.

Examples

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data(Simon)
# Background Correction
c.spot <- bg.correct(Simon)
#Normalized data
n.spot <- global.norm(c.spot)

Example output

Loading required package: locfit
locfit 1.5-9.1 	 2013-03-22
Loading required package: tkrplot
Loading required package: tcltk

Attaching package: 'genArise'

The following object is masked from 'package:utils':

    help

Warning messages:
1: no DISPLAY variable so Tk is not available 
2: loading Rplot failed 

genArise documentation built on Nov. 8, 2020, 6:29 p.m.