stat.deseq: Statistical testing with DESeq

In metaseqR: An R package for the analysis and result reporting of RNA-Seq data by combining multiple statistical algorithms.

Description

This function is a wrapper over DESeq statistical testing. It accepts a matrix of normalized gene counts or an S4 object specific to each normalization algorithm supported by metaseqR.

Usage

    stat.deseq(object, sample.list, contrast.list = NULL,
        stat.args = NULL)

Arguments

object	a matrix or an object specific to each normalization algorithm supported by metaseqR, containing normalized counts. Apart from matrix (also for NOISeq), the object can be a SeqExpressionSet (EDASeq), CountDataSet (DESeq) or DGEList (edgeR).
sample.list	the list containing condition names and the samples under each condition.
contrast.list	a named structured list of contrasts as returned by make.contrast.list or just the vector of contrasts as defined in the main help page of metaseqr.
stat.args	a list of DESeq statistical algorithm parameters. See the result of get.defaults("statistics", "deseq") for an example and how you can modify it. It is not required when the input object is already a CountDataSet from DESeq normalization as the dispersions are already estimated.

Value

A named list of p-values, whose names are the names of the contrasts.

Author(s)

Panagiotis Moulos

Examples

require(DESeq)
data.matrix <- counts(makeExampleCountDataSet())
sample.list <- list(A=c("A1","A2"),B=c("B1","B2","B3"))
contrast <- "A_vs_B"
norm.data.matrix <- normalize.deseq(data.matrix,sample.list)
p <- stat.deseq(norm.data.matrix,sample.list,contrast)

metaseqR documentation built on Nov. 8, 2020, 5:57 p.m.