hi_loadings: Extract genes with highest loadings

In pcaExplorer: Interactive Visualization of RNA-seq Data Using a Principal Components Approach

Description

Extract genes with highest loadings

Usage

hi_loadings(
  pcaobj,
  whichpc = 1,
  topN = 10,
  exprTable = NULL,
  annotation = NULL,
  title = "Top/bottom loadings"
)

Arguments

pcaobj	A prcomp object
whichpc	An integer number, corresponding to the principal component of interest
topN	Integer, number of genes with top and bottom loadings
exprTable	A matrix object, e.g. the counts of a DESeqDataSet. If not NULL, returns the counts matrix for the selected genes
annotation	A data.frame object, with row.names as gene identifiers (e.g. ENSEMBL ids) and a column, gene_name, containing e.g. HGNC-based gene symbols
title	The title of the plot

Value

A ggplot2 object, or a matrix, if exprTable is not null

Examples

dds <- makeExampleDESeqDataSet_multifac(betaSD = 3, betaSD_tissue = 1)
rlt <- DESeq2::rlogTransformation(dds)
pcaobj <- prcomp(t(SummarizedExperiment::assay(rlt)))
hi_loadings(pcaobj, topN = 20)
hi_loadings(pcaobj, topN = 10, exprTable = dds)
hi_loadings(pcaobj, topN = 10, exprTable = counts(dds))

pcaExplorer documentation built on Nov. 8, 2020, 5:29 p.m.