NormalizeProbes: Normalize tiling array data using sequence information

In rMAT: R implementation from MAT program to normalize and analyze tiling arrays and ChIP-chip data.

Description

This function is used to normalize tiling array data using sequence information. Users can chose between two different normalization methods. Please refer to the arguments section below.

Usage

NormalizeProbes(tilingSet, method="MAT", robust=FALSE,
                all=FALSE, standard=TRUE, verbose=FALSE)
						

Arguments

tilingSet	This object contains an ExpressionSet and has the following additional slots
method	The normalization method to be used. User can choose from "MAT", or "PairBinned". As an upgrade to MAT, the Pair option also takes into account of the interaction between adjacent pairs along the probe as covariates for linear regression.
robust	A logical value. If TRUE, reweighted least-squares estimates are computed.
all	A logical value. If not using all probes to compute (for faster computation and memory efficiency) the regression parameters, then use the minimum of 300,000 or number of probes, whichever is less.
standard	Typical methods.
verbose	A logical value. If verbose is TRUE, progress information would be displayed.

Details

For the original rMAT normalization: method is set to be rMAT in string, robust is set to be false, copyNumber is set to be your copy number's vector, rMATScaling is set to be true, and logTransform is set to be true for untransformed data. The output can be saved as BAR file if the BAR argument specifies a filename, or as a parsed BAR file if argument output specifies a filename.

For more details on normalization, please refer to the following paper: Johnson et al. Model-based analysis of tiling-arrays for ChIP-chip. Proc Natl Acad Sci USA (2006) vol. 103 (33) pp. 12457-62

Value

The matrix of normalized expression values.

Author(s)

Charles Cheung, [e-mail] and Raphael Gottardo, [e-mail] Arnaud Droit, [e-mail]

See Also

PairInMatrix() for generating neighbouring pair-codes from sequences and affyTile for information about the package.

Examples

####################################################
#The data are in inst/doc folder in rMAT package.
####################################################



pwd<-"" #INPUT FILES- BPMAP, ARRAYS, etc.
path<- system.file("extdata", "Sc03b_MR_v04_10000.bpmap",package="rMAT")

bpmapFile<-paste(pwd,path,sep="")

pathCEL<- system.file("extdata", "Swr1WTIP_Short.CEL",package="rMAT")
arrayFile<-paste(pwd,c(pathCEL),sep="")


# Show the all the different sequences
ReadBPMAPAllSeqHeader(bpmapFile)

# create a tiling Set from the corresponding data
# This will only grep the sequences with Sc

ScSet <- BPMAPCelParser(bpmapFile, arrayFile, verbose=FALSE, groupName="Sc") 
ScSetNorm <- NormalizeProbes(ScSet, method="MAT", robust=FALSE, all=FALSE,
    standard=TRUE, verbose=FALSE)
   

rMAT documentation built on May 6, 2019, 2:10 a.m.