Data Analyses

In recountmethylation: Access and Analyze DNA Methylation Array Databases

suppressMessages(library(rhdf5))
suppressMessages(library(minfi))
suppressMessages(library(recountmethylation))
suppressMessages(library(knitr))
suppressMessages(library(ggplot2))
suppressMessages(library(gridExtra))
suppressMessages(library(GenomicRanges))
suppressMessages(library(limma))
suppressMessages(library(HDF5Array))
opts_chunk$set(eval = FALSE, echo = TRUE, 
               warning = FALSE, message = FALSE)

Overview

This vignette walks through 3 analysis examples using data accessed with the recountmethylation package. First, predicted and chronological ages are compared from the sample metadata. Then quality signals (methylated and unmethylated, log2 median scale) are compared between samples stored using either formalin fixed paraffin-embedding (FFPE) or freezing. Finally, tissue-specific probe sets with high DNA methylation (DNAm) fraction variances are identifed and analyzed using liver and adipose samples. Note that versions of these analyses also appear in the manuscript @maden_human_2020.

Analysis script and limited chunk evaluation

This vignette accompanies the "data_analyses.R" script. Note the script was written with extensibility to new and larger comparator groups in mind. While the script should run to completion without errors, it takes several hours in total to complete (excluding the time to download large database files). Due to this lengthy script run time, this vignette only evaluates code chunks utilizing final/resultant data objects produced by the script (e.g. for tables, tests, and figures). For completeness, remaining script steps and code are included but not evaluated.

Load the file "data_analyses.RData" from the recountmethylation package files. This contains the resultant/final data objects produced by the script, which will be used in evaluated code chunks below.

sf <- system.file(file.path("extdata", "data_analyses"), 
                  package = "recountmethylation")
load(file.path(sf, "data_analyses.RData"))

Datasets and data objects

The analysis script uses sample metadata and 2 database files. Retrieve the provided sample metadata from the recountmethylation package files.

# get local metadata
path <- system.file("extdata", "metadata", package = "recountmethylation")
mdpath <- paste(path, list.files(path)[1], sep = "/")
md <- get(load(mdpath))

Also obtain 2 HDF5-SummarizedExperiment database files, the GenomicRanges and MethylSet files. Consult the users_guide vignette for details about the database file formats and download instructions. Once the datasets downloaded, they can be loaded into an R session as follows.

# load methylset
gmdn <- "remethdb-h5se_gm_0-0-1_1590090412"
gm <- loadHDF5SummarizedExperiment(gmdn)
# load grset
grdn <- "remethdb-h5se_gr_0-0-1_1590090412"
gr <- loadHDF5SummarizedExperiment(grdn)

Example 1: Comparing mined and predicted age

This example uses sample metadata to compare mined and predicted ages from the age and predage variables, respectively. Values in age were mined from GEO record metadata and are included with available age units. Values in predage were calculated from noob-normalized (@triche_low-level_2013) DNAm Beta-values with agep, a function from the wateRmelon package that implements the Horvath biological age clock (@horvath_dna_2013).

Make new variables and filter samples

Get samples for which both age and predage age are available. From age, make a new numeric variable chron.age.

mdf <- md[!md$age == "valm:NA",]
mdf$chron.age <- as.numeric(gsub(";.*", "", gsub("^valm:", "", mdf$age)))
mdf$predage <- as.numeric(mdf$predage)
mdf <- mdf[!is.na(mdf$chron.age),]
mdf <- mdf[!is.na(mdf$predage),]

Next, make a new variable stype from sampletype and remove samples with missing values.

mdf$stype <- as.character(gsub(";.*", "", 
  gsub("^msraptype:", "", mdf$sampletype)))
mdf <- mdf[!is.na(mdf$stype),]

Now make a new variable is.cx from querying cancer in the disease term. This reflects whether a sample was likely from a cancer or a cancer patient.

mdf$is.cx <- ifelse(grepl(".*cancer.*", mdf$disease), TRUE, FALSE)

Next, store the study-wise age differences in the xdif variable using the mean absolute difference (a.k.a. "MAD") between chron.age and predage across samples from the same study. Also store study sizes in the ngsm term for plotting.

xdif <- ngsm <- c()
for(g in unique(mdf$gseid)){
    mdff <- mdf[mdf$gseid==g, ]
    xdif <- c(xdif, mean(abs(mdff$chron.age - as.numeric(mdff$predage))))
    ngsm <- c(ngsm, nrow(mdff))
}
names(xdif) <- names(ngsm) <- unique(mdf$gseid)

Make a new filtered mdff data frame using the new variables. Retain likely non-cancer samples from studies with MAD <= 10 years. Pre- and post-filter datasets (groups 1 and 2, respectively) are summarized below.

filt <- mdf$stype == "tissue" & !mdf$is.cx
filt <- filt & !mdf$gseid %in% names(xdif[xdif > 10])
mdff <- mdf[filt, ]

Analyses and summary statistics

Perform statistical analyses of mdf (group 1) and mdff (group 2). First, generate multiple regressions for each.

lm1 <- lm(mdf$predage ~ mdf$chron.age + mdf$gseid + mdf$stype + mdf$is.cx)
lm2 <- lm(mdff$predage ~ mdff$chron.age + mdff$gseid)

Now perform analyses of variances (ANOVAs) on multiple regressions. Summarize variance percentages and p-values for covariates in each model. Columns "Vperc" and "Pval" are the percent variance and unadjusted p-value for covariates in each model.

# anovas
av1 <- anova(lm1)
av2 <- anova(lm2)
# results summaries
sperc1 <- round(100*av1$`Sum Sq`[1:4]/sum(av1$`Sum Sq`), 2)
pval1 <- format(av1$`Pr(>F)`[1:4], scientific = TRUE, digits = 3)
sperc2 <- round(100*av2$`Sum Sq`[1:2]/sum(av2$`Sum Sq`), 2)
pval2 <- format(av2$`Pr(>F)`[1:2], scientific = TRUE, digits = 3)
# summary table
dan <- data.frame(Vperc1 = c(sperc1), 
                  Pval1 = c(pval1),
                  Vperc2 = c(sperc2, "-", "-"), 
                  Pval2 = c(pval2, "-", "-"), 
                  stringsAsFactors = FALSE)
rownames(dan) <- c("Chron.Age", "GSEID", "SampleType", "Cancer")
knitr::kable(dan, align = "c")

Now calcualte the R-squared, Spearman correlation coefficient (Rho), and MAD for each model.

# rsquared
rsq1 <- round(summary(lm1)$r.squared, 2)
rsq2 <- round(summary(lm2)$r.squared, 2)
# correlation coefficient
rho1 <- round(cor.test(mdf$predage, mdf$chron.age, 
                      method = "spearman")$estimate, 2)
rho2 <- round(cor.test(mdff$predage, mdff$chron.age, 
                       test = "spearman")$estimate, 2)
# mean absolute difference
mad1 <- round(mean(abs(mdf$chron.age - mdf$predage)), 2)
mad2 <- round(mean(abs(mdff$chron.age - mdff$predage)), 2)

Finally, organize and display the results

dss <- data.frame(group = c("1", "2"),
                  ngsm = c(nrow(mdf), nrow(mdff)),
                  ngse = c(length(unique(mdf$gseid)), 
                    length(unique(mdff$gseid))),
                  r.squared = c(rsq1, rsq2), rho = as.character(c(rho1, rho2)),
                  mad = c(mad1, mad2), stringsAsFactors = FALSE)
knitr::kable(dss, align = "c")

Scatter plots of study errors and sample ages

Plot sample counts and MAD for each GSE record, with a vertical line at the 10-years MAD cutoff used for the group 2 filter.

plot(xdif, ngsm, ylab = "Study Size (Num. GSM)", 
     xlab = "Age Difference, MAD[Chron, Pred]")
abline(v = 10, col = "red")

Finally, plot the chronological and predicted ages for group 2 samples.

ggplot(mdff, aes(x = chron.age, y = predage)) +
  geom_point(size = 1.2, alpha = 0.2) + geom_smooth(method = "lm", size = 1.2) +
  theme_bw() + xlab("Chronological Age") + ylab("Epigenetic (DNAm) Age")

Example 2: Signal comparison of FFPE and frozen samples

This section compares methylated and unmethylated signal (log2 sample median scale) between samples stored with either FFPE or fresh freezing (FF).

Get samples with storage type information

Identify and summarize samples with the storage variable available. Use values in storage to inform a new sgroup variable.

mdf <- md[!md$storage == "NA",]
mdf$sgroup <- ifelse(grepl("FFPE", mdf$storage), "ffpe", "frozen")

# get summary table
sst <- get_sst(sgroup.labs = c("ffpe", "frozen"), mdf)
knitr::kable(sst, align = "c") # table display

Use blocking to calculate signal log2 medians

Subset the MethylSet object and extract the full signal matrices with the getMeth and getUnmeth functions from the minfi package.

gmf <- gm[, gm$gsm %in% mdf$gsm] # filt h5se object
mdf <- mdf[order(match(mdf$gsm, gmf$gsm)),]
identical(gmf$gsm, mdf$gsm)
gmf$storage <- mdf$storage # append storage info

meth.all <- getMeth(gmf)
unmeth.all <- getUnmeth(gmf)

Next, prepare to calculate log2 median signals. To manage data in active memory, process it in smaller units or blocks. Using the get_blocks helper function, assign sample indices to blocks of size 1,000 using the bsize argument.

blocks <- getblocks(slength = ncol(gmf), bsize = 1000)

Now calculate log2 of sample median signals for each block. Vectorize calculations within blocks with apply. Store results in the data.frame ds.

ms <- matrix(nrow = 0, ncol = 2)
l2meth <- l2unmeth <- c()
for(i in 1:length(blocks)){
  b <- blocks[[i]]
  gmff <- gmf[, b]
  methb <- as.matrix(meth.all[, b])
  unmethb <- as.matrix(unmeth.all[, b])
  l2meth <- c(l2meth, apply(methb, 2, function(x){
    log2(median(as.numeric(x)))
  }))
  l2unmeth <- c(l2unmeth, apply(unmethb, 2, function(x){
    log2(median(as.numeric(x)))
  }))
  ms <- rbind(ms, matrix(c(l2meth, l2unmeth), ncol = 2))
  message(i)
}
rownames(ms) <- colnames(meth.all)
colnames(ms) <- c("meth.l2med", "unmeth.l2med")
ds <- as.data.frame(ms)
ds$storage <- ifelse(grepl("FFPE", gmf$storage), "ffpe", "frozen")

Signals plotted by storage type

Evaluate signal patterns across storage type using plots using the ggplot2 package. First, make a 2d scatter plot of methylated and unmethylated signals using the geom_point function. Color by storage type with the scale_color_manual function (FFPE samples are orange, frozen samples are purple).

ggplot(ds, aes(x = meth.l2med, y = unmeth.l2med, color = storage)) + 
  geom_point(alpha = 0.35, cex = 3) + theme_bw() +
  scale_color_manual(values = c("ffpe" = "orange", "frozen" = "purple"))

Next, make separate violin plots for signals and groups using the geom_violin function with the same colors for each storage type. Draw horizontal median lines by setting the draw_quantiles argument to 0.5.

vp <- matrix(nrow = 0, ncol = 2)
vp <- rbind(vp, matrix(c(ds$meth.l2med, paste0("meth.", ds$storage)), 
  ncol = 2))
vp <- rbind(vp, matrix(c(ds$unmeth.l2med, paste0("unmeth.", ds$storage)), 
  ncol = 2))
vp <- as.data.frame(vp, stringsAsFactors = FALSE)
vp[,1] <- as.numeric(vp[,1])
colnames(vp) <- c("signal", "group")
vp$col <- ifelse(grepl("ffpe", vp$group), "orange", "purple")
# make plot
ggplot(vp, aes(x = group, y = signal, color = group)) + 
  scale_color_manual(values = c("meth.ffpe" = "orange", 
    "unmeth.ffpe" = "orange", "meth.frozen" = "purple", 
    "unmeth.frozen" = "purple")) +
  geom_violin(draw_quantiles = c(0.5)) + theme_bw() + 
    theme(legend.position = "none")

Example 3: Identify and analyze tissue-specific probes with the highest

variances

This example describes variance analyses in liver and adipose, 2 of the 7 tissues analyzed in the manuscript @maden_human_2020. This includes a quality assessment, study ID linear adjustment of DNAm fractions, ANOVA-based and probe filtering, 2-step variance analyses, and results plots.

Sample identification and summary

Summarize the samples of interest. Use two vectors of GSM IDs, adipose.gsmv and liver.gsmv to filter the metadata (see vectors in the data_analyses.R script). Also define tissues in the new group variable sgroup. Summarize the sample groups in a table

gsmv <- c(adipose.gsmv, liver.gsmv)
mdf <- md[md$gsm %in% gsmv,]
mdf$sgroup <- ifelse(mdf$gsm %in% adipose.gsmv, "adipose", "liver")
sst.tvar <- get_sst(sgroup.labs = c("liver", "adipose"), mdf)
knitr::kable(sst.tvar, align = "c")

Calculate log2 methylated and unmethylated signal medians

Subset the MethylSet dataset, then append the sgroup variable from mdf and map the object to the genome using the mapToGenome function from the minfi package.

ms <- gm[,colnames(gm) %in% rownames(mdf)]
ms <- ms[,order(match(colnames(ms), rownames(mdf)))]
identical(colnames(ms), rownames(mdf))
# [1] TRUE
ms$sgroup <- mdf$sgroup
ms <- mapToGenome(ms)
dim(ms)
# [1] 485512    252

As in example 2 above, calculate the sample log2 median signals from signal matrices. Process the data in blocks using within-block vectorization with apply.

# get log2 medians
meth.tx <- getMeth(ms)
unmeth.tx <- getUnmeth(ms)
blocks <- getblocks(slength = ncol(ms), bsize = 50)
# process data in blocks
l2m <- matrix(nrow = 0, ncol = 2)
for(i in 1:length(blocks)){
  b <- blocks[[i]]
  gmff <- ms[, b]
  methb <- as.matrix(meth.tx[, b])
  unmethb <- as.matrix(unmeth.tx[, b])
  l2meth <- l2unmeth <- c()
  l2meth <- c(l2meth, apply(methb, 2, function(x){
    log2(median(as.numeric(x)))
  }))
  l2unmeth <- c(l2unmeth, apply(unmethb, 2, function(x){
    log2(median(as.numeric(x)))
  }))
  l2m <- rbind(l2m, matrix(c(l2meth, l2unmeth), ncol = 2))
  message(i)
}
ds2 <- as.data.frame(l2m)
colnames(ds2) <- c("l2med.meth", "l2med.unmeth")
ds2$tissue <- as.factor(ms$sgroup)

Make a scatter plot of log2 median signals by tissue type with the geom_point function.

ggplot(ds2, aes(x = l2med.meth, y = l2med.unmeth, color = tissue)) + 
  geom_point(alpha = 0.3, cex = 3) + theme_bw()

Perform linear correction on DNAm for study IDs

Access the noob-normalized DNAm Beta-values from the GenomicRatio object gr loaded above. Extract the DNAm fractions as M-values (logit2 transformed Beta-values) with the getM minfi function. Perform linear correction on study ID with the removeBatchEffect function from the limma package by setting the batch argument to the "gseid" variable.

lmv <- lgr <- lmd <- lb <- lan <- list()
tv <- c("adipose", "liver")
# get noob norm data
gr <- gr[,colnames(gr) %in% colnames(ms)]
gr <- gr[,order(match(colnames(gr), colnames(ms)))]
identical(colnames(gr), colnames(ms))
gr$sgroup <- ms$sgroup
# do study ID adj
for(t in tv){
  lmv[[t]] <- gr[, gr$sgroup == t]
  msi <- lmv[[t]]
  madj <- limma::removeBatchEffect(getM(msi), batch = msi$gseid)
  # store adjusted data in a new se object
  lgr[[t]] <- GenomicRatioSet(GenomicRanges::granges(msi), M = madj, 
                              annotation = annotation(msi))
  # append samples metadata
  lmd[[t]] <- pData(lgr[[t]]) <- pData(lmv[[t]])
  # append preprocessing metadata
  metadata(lgr[[t]]) <- list("preprocess" = "noobbeta;removeBatchEffect_gseid")
  # make betavals list
  lb[[t]] <- getBeta(lgr[[t]]) # beta values list
}

Perform array-wide ANOVAs and filter probes

Prepare and run ANOVAs on autosomal probes. First, identify and remove sex chromosome probes by accessing annotation with the getAnnotation minfi function. List the filtered data in the lbf object.

anno <- getAnnotation(gr)
chr.xy <-c("chrY", "chrX")
cg.xy <- rownames(anno[anno$chr %in% chr.xy,])
lbf <- list()
for(t in tv){
  bval <- lb[[t]]
  lbf[[t]] <- bval[!rownames(bval) %in% cg.xy,]
}
bv <- lbf[[1]]

Next, select and format the 9 model covariates for the ANOVA tests. From sample metadata, select the variables for study ID ("gseid"), predicted sex ("predsex"), predicted age ("predage"), and predicted fractions of 6 cell types ("predcell..*"). Convert these to either factor or numeric type with the functions as.factor and as.numeric, respectively.

lvar <- list()
cnf <- c("gseid", "predsex", "predage", "predcell.CD8T",
         "predcell.CD4T", "predcell.NK", "predcell.Bcell",
         "predcell.Mono", "predcell.Gran")
for(t in tv){
  for(c in cnf){
    if(c %in% c("gseid", "predsex")){
      lvar[[t]][[c]] <- as.factor(pData(lgr[[t]])[,c])
    } else{
      lvar[[t]][[c]] <- as.numeric(pData(lgr[[t]])[,c])
    }
  }
}

Run ANOVAs on probe Beta-values. Use the blocking-with-vectorization strategy here as above, with large blocks of 100,000 sample indices each. Calculations should complete in about 1 hour. For each test, retain unadjusted p-values and variance percentages of the 9 covariates. Store the 18-column results matrices in the lan list object.

bv <- lbf[[1]]
blocks <- getblocks(slength = nrow(bv), bsize = 100000)
mr <- matrix(nrow = 0, ncol = 18)
lan <- list("adipose" = mr, "liver" = mr)
t1 <- Sys.time()
for(bi in 1:length(blocks)){
  for(t in tv){
    datr <- lbf[[t]][blocks[[bi]],]
    tvar <- lvar[[t]]
    newchunk <- t(apply(datr, 1, function(x){
      # do multiple regression and anova
      x <- as.numeric(x)
      ld <- lm(x ~ tvar[[1]] + tvar[[2]] + tvar[[3]] + tvar[[4]] +
                 tvar[[5]] + tvar[[6]] + tvar[[7]] + tvar[[8]] + tvar[[9]])
      an <- anova(ld)
      # get results
      ap <- an[c(1:9),5] # pval
      av <- round(100*an[c(1:9),2]/sum(an[,2]), 3) # percent var
      return(as.numeric(c(ap, av)))
    }))
    # append new results
    lan[[t]] <- rbind(lan[[t]], newchunk)
  }
  message(bi, "tdif: ", Sys.time() - t1)
}
# append colnames
for(t in tv){colnames(lan[[t]]) <- rep(cnf, 2)}

Next, remove probes showing evidence of residual confounding from the covariates. Adjust covariate p-values with the p.adjust function, and retain probes with adjusted p-values >= 0.001 and variance < 10% variance for all 9 covariates. Retain the filtered probe DNAm data as GenomicRatioSets for each tissue in the list lgr.filt.

pfilt <- 1e-3
varfilt <- 10
lcgkeep <- list() # list of filtered probe sets
for(t in tv){
  pm <- lan[[t]][,c(1:9)]
  vm <- lan[[t]][,c(10:18)]
  # parse variable thresholds
  cm <- as.data.frame(matrix(nrow = nrow(pm), ncol = ncol(pm)))
  for(c in 1:ncol(pm)){
    pc <- pm[,c]; 
    pc.adj <- as.numeric(p.adjust(pc))
    pc.filt <- pc.adj < pfilt
    vc.filt <- vm[,c] >= varfilt
    cm[,c] <- (pc.filt & vc.filt)
  }
  cgkeep <- apply(cm, 1, function(x){return((length(x[x == TRUE]) == 0))})
  lcgkeep[[t]] <- rownames(pm)[cgkeep]
}
lgr.filt <- list("adipose" = lgr[[1]][lcgkeep[[1]],],
                 "liver" = lgr[[2]][lcgkeep[[2]],])

Get probe DNAm summary statistics and analyze variances

Calculate probe DNAm summary statistics. For each tissue, calculate the minima, maxima, means, medians, standard deviations, and variances of Beta-values across samples. Store results in the lcg.ss list.

cnv <- c("min", "max", "mean", "median", "sd", "var")
bv <- getBeta(lgr.filt[[t]])
lbt <- lcg.ss <- list()
bsize = 100000
for(t in tv){
  lcg.ss[[t]] <- matrix(nrow = 0, ncol = 6)
  lbt[[t]] <- bt <- as.matrix(getBeta(lgr.filt[[t]]))
  blockst <- getblocks(slength = nrow(bt), bsize = bsize)
  for(bi in 1:length(blockst)){
    bc <- bt[blockst[[bi]],]
    newchunk <- t(apply(bc, 1, function(x){
      newrow <- c(min(x), max(x), mean(x), median(x), sd(x), var(x))
      return(as.numeric(newrow))
    }))
    lcg.ss[[t]] <- rbind(lcg.ss[[t]], newchunk)
    message(t, ";", bi)
  }
  colnames(lcg.ss[[t]]) <- cnv
}

Perform the main variance analyses with 2 strategies. This selects the 2,000 probes with the highest group-specific variances.

First, use a single variance cutoff, or "absolute" quantile cutoff, for each group. List probes in the top 99th quantile variances for each tissue in the lmvp.abs object.

qiv = seq(0, 1, 0.01)
qwhich = c(100)
lmvp.abs <- list()
lci <- list()
for(t in tv){
  cgv <- c()
  sa <- lcg.ss[[t]]
  sa <- as.data.frame(sa, stringsAsFactors = FALSE)
  q <- quantile(sa$var, qiv)[qwhich]
  lmvp.abs[[t]] <- rownames(sa[sa$var > q,])
}

Now select high-variance probes with binning for each tissue. Assign probes to 1 of 10 bins using 0.1 mean Beta-value intervals. Select probes in the top 99th variance quantiles for each bin, and store in lmvp.bin.

# binned quantiles method
qiv = seq(0, 1, 0.01) # quantile filter
qwhich = c(100)
bin.xint <- 0.1
binv = seq(0, 1, bin.xint)[1:10] # binned bval mean
# iter on ncts
lmvp.bin = list()
for(t in tv){
  sa <- as.data.frame(lcg.ss[[t]])
  cgv <- c()
  # iterate on betaval bins
  for(b in binv){
    bf <- sa[sa$mean >= b & sa$mean < b + bin.xint, ] # get probes in bin
    q <- qf <- quantile(bf$var, qiv)[qwhich] # do bin filter
    cgv <- c(cgv, rownames(bf)[bf$var > q]) # append probes list
  }
  lmvp.bin[[t]] <- cgv
}

With the variance analyses complete, filter the lmvp.abs and lmvp.bin probes by tissue specificity. Tissue-specific probes should only occur among high variance probes for a single tissue. Categorize probes as "tissue-specific" or "non-specific" using the table function to determine their frequency of occurrence across tissues.

cgav <- c()
for(t in tv){
  txcg <- unique(c(lmvp.abs[[t]], lmvp.bin[[t]]))
  cgav <- c(cgav, txcg)
}
cgdf <- as.data.frame(table(cgav))
cgdf$type <- ifelse(cgdf[,2] > 1, "non-specific", "tissue-specific")
table(cgdf$type)

After filtering probes by tissue specificity, rank them by descending DNAm variance. Select 1,000 probes from lmvp.abs, then 1,000 non-overlapping probes from lmvp.bin, retain the 2,000 highest-variance probes by tissue in the ltxcg list.

cgfilt <- cgdf$type == "non-specific"
cgdff <- cgdf[!cgfilt,]
ltxcg <- list()
for(t in tv){
  cgtx <- c()
  cgabs <- lmvp.abs[[t]]
  cgbin <- lmvp.bin[[t]]
  st <- as.data.frame(lcg.ss[[t]])
  # get t tissue specific probes
  filtbt <- rownames(st) %in% cgdff[,1]
  st <- st[filtbt,]
  # get top 1k t tissue specific abs probes
  filt.bf1 <- rownames(st) %in% cgabs
  sf1 <- st[filt.bf1,]
  sf1 <- sf1[rev(order(sf1$var)),]
  cgtx <- rownames(sf1)[1:1000]
  # get top 1k t tissue specific bin probes, after filt
  filt.bf2 <- rownames(st) %in% cgbin &
              !rownames(st) %in% rownames(sf1)
  sf2 <- st[filt.bf2,]
  sf2 <- sf2[rev(order(sf2$var)),]
  cgtx <- c(cgtx, rownames(sf2)[1:1000])
  ltxcg[[t]] <- cgtx
}

Violin plots and heatmaps of probe set DNAm means and variances

First, get probe set DNAm summaries and annotation data.

# filtered cg summaries
lfcg <- lapply(lcg.ss, 
  function(x){x <- x[rownames(x) %in% unique(unlist(ltxcg)),]})
# annotation subset
anno <- getAnnotation(gr) # save anno for cga
anno <- anno[,c("Name", "UCSC_RefGene_Name", "UCSC_RefGene_Group", 
  "Relation_to_Island")]
anno <- anno[rownames(anno) %in% unique(unlist(ltxcg)),]
# filtered beta values
lcgssf <- list()
for(t in tv){
  bv <- lcg.ss[[t]]
  bvf <- bv[rownames(bv) %in% ltxcg[[t]],]
  lcgssf[[t]] <- bvf
}

Use the makevp() helper function to make violin plots with horizontal bars at distribution medians. This function formats the data and calls geom_violin to make violin plots for DNAm fraction means and variances. Store plots in the lvp list, then display them vertically using the grid.arrange function from the gridExtra package.

lvp <- makevp(lfcg, ltxcg)
grid.arrange(lvp[[1]], lvp[[2]], ncol = 1, bottom = "Tissue")

Tabulate the means of probe set statistics by tissue.

tcgss <- matrix(nrow = 0, ncol = 6)
for(t in tv){
  datt <- apply(lcgssf[[t]], 2, function(x){
      round(mean(x), digits = 2)
  })
  mt <- matrix(datt, nrow = 1)
  tcgss <- rbind(tcgss, mt)
}
colnames(tcgss) <- colnames(lcgssf$adipose)
rownames(tcgss) <- tv
knitr::kable(t(tcgss), align = "c")

Next, prepare genome region heatmaps with 3 helper functions. These will tabulate probe abundances by region and use the probe Beta-value means to calculate the mean of means (left heatmap) and variance of means (right heatmap) by genome region type.

First, define island and gene annotation groups from the manifest using get_cga. Next, get region-specific DNAm summaries with hmsets using a minimum region coverage of 2. This means values are calculated for regions with least 2 probes, and regions with less are assigned "NA" and greyed out. Make the 2 plots objects for means and variances with hmplots(), which wraps the geom_tile ggplot2 function. Finally, display plots horizontally with grid.arrange.

cga <- get_cga(anno)
lhmset <- hmsets(ltxcg, lfcg, cga)
lhmplots <- hmplots(lhmset$hm.mean, lhmset$hm.var, lhmset$hm.size)
grid.arrange(lhmplots$hm.mean.plot, lhmplots$hm.var.plot, 
             layout_matrix = matrix(c(1, 1, 1, 1, 1, 2, 2), nrow = 1),
             bottom = "Tissue", left = "Annotation/Region Type")

Colors blue, white, and red represent low, intermediate, and high means and variances of region-specific mean Beta-values. Cell numbers show probe region quantities and are identical for both plots.

Conclusions

This vignette described cross-study analyses using data objects accessible with recountmethylation and appearing in the manuscript @maden_human_2020. See the manuscript for more information about samples, quality metric signal patterns, and extended variability analyses. For details about data objects, consult the package users_guide vignette. Full code and helper function definitions are contained in the data_analyses.R companion script. For additional utilities to analyze DNAm data, consult the minfi and wateRmelon packages.

Session info

sessionInfo()

Works Cited

recountmethylation documentation built on Nov. 8, 2020, 4:59 p.m.