shiftCor_dQC: QC-free based signal correction

Description Usage Arguments Value Examples

View source: R/shiftCor_combat.R

Description

shiftCor_dQC provides the QC-free based signal correction for large scale mass spectrometry-based omics data.

Usage

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shiftCor_dQC(samPeno, samFile, Frule = 0.8, imputeM = "KNN",
  MLmethod = "Combat", par.prior = TRUE, prior.plots = FALSE,
  mod.covariates = FALSE, batch.Num = NULL)

Arguments

samPeno

The file with the meta information including the sample name, batches, class and order (denoting other covariates besides batch).

samFile

The file with the expression information.

Frule

Modified n precent rule function. A variable will be kept if it has a non-zero value for at least n precent of samples in any one group. (Default: 0.8)

imputeM

The parameter for imputation method i.e., nearest neighbor averaging, 'KNN'; minimum values, 'min'; Half of minimum values, 'minHalf'; median values, 'median'.

MLmethod

'ComBat' allows users to adjust for batch effects in datasets where the batch covariate is known, using methodology described in Johnson et al. 2007. It uses either parametric or non-parametric empirical Bayes frameworks for adjusting data for batch effects. Users are returned an expression matrix that has been corrected for batch effects.The function was revised accroding to 'sva' package (version = "3.8").

par.prior

TRUE indicates parametric adjustments will be used, FALSE indicates non-parametric adjustments will be used

prior.plots

(Optional) TRUE give prior plots.

mod.covariates

TRUE indicates model matrix for outcome of interest and other covariates besides batch (Column 'order' denotes covariates the in samPeno file).

batch.Num

(Optional) NULL If given, will use the selected batch as a reference for batch adjustment.

Value

the shiftCor files. See the details at https://stattarget.github.io

Examples

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datpath <- system.file('extdata',package = 'statTarget')
samPeno <- paste(datpath,'MTBLS79_dQC_sampleList.csv', sep='/')
samFile <- paste(datpath,'MTBLS79.csv', sep='/')
shiftCor_dQC(samPeno,samFile, Frule = 0.8, MLmethod = "Combat",mod.covariates = FALSE)
shiftCor_dQC(samPeno,samFile, Frule = 0.8, MLmethod = "Combat",mod.covariates = TRUE,batch.Num = 1)

statTarget documentation built on Nov. 8, 2020, 8:27 p.m.