dot-renormaliseByChannel: Normalise a well on one channel only and then transform it...

In twoddpcr: Classify 2-d Droplet Digital PCR (ddPCR) data and quantify the number of starting molecules

Description

Normalise a well on one channel only and then transform it back to the original (combined) scale.

Usage

.renormaliseByChannel(wellDf, combinedCentres, wellCentres, channel)

Arguments

wellDf	A data frame of the well's droplet amplitudes.
combinedCentres	A data frame of the combined (average) centres of the non-normalised wells.
wellCentres	A data frame of centres corresponding to the given channel.
channel	An integer 1 or 2 corresponding to the channel that we are interested in.

Value

A data frame with the rescaled amplitudes in the chosen channel.

Author(s)

Anthony Chiu, [e-mail]

twoddpcr documentation built on Nov. 8, 2020, 5:49 p.m.