startedLog: prepares OTU counts data for PCA analysis using started-log...

Description Usage Arguments Value Author(s) References

View source: R/startedLog.R

Description

Normalizes counts based on total counts per sample, adds a constant number (argument logstart) to get rid of zeroes, then log-transforms the data.

Usage

1
startedLog(data,count.columns,logstart=0.1)

Arguments

data

OTU counts table. Rows: samples, columns: OTUs.

count.columns

numbers of colums containing OTU counts.

logstart

number to add to zero counts before log transform. With 'logstart=0', the zero count datapoints become 'NA' (useful for analysis of pairwise correlations between OTUs).

Value

A table of log-transformed normalized values.

Author(s)

Mikhail V. Matz, University of Texas at Austin <matz@utexas.edu>

References

Started Log transform: Rocke DM and Durbin B Bioinformatics 2003 19:966-972 (DOI: 10.1093/bioinformatics/btg107) MCMC.OTU: Elizabeth A. Green, Sarah W. Davies, Mikhail V. Matz, Monica Medina Next-generation sequencing reveals cryptic Symbiodinium diversity within Orbicella faveolata and Orbicella franksi at the Flower Garden Banks, Gulf of Mexico. PeerJ 2014 https://peerj.com/preprints/246/


MCMC.OTU documentation built on May 1, 2019, 10:55 p.m.