ancombc_pq: Run ANCOMBC2 on phyloseq object

In MiscMetabar: Miscellaneous Functions for Metabarcoding Analysis

Run ANCOMBC2 on phyloseq object

Description

A wrapper for the ANCOMBC::ancombc2() function

Usage

ancombc_pq(physeq, fact, levels_fact = NULL, tax_level = "Class", ...)

Arguments

physeq	(required): a phyloseq-class object obtained using the phyloseq package.
fact	(required) Name of the factor in physeq@sam_data used to plot different lines
levels_fact	(default NULL) The order of the level in the factor. Used for reorder levels and select levels (filter out levels not present en levels_fact)
tax_level	The taxonomic level passed on to ANCOMBC::ancombc2()
...	Other arguments passed on to ANCOMBC::ancombc2() function.

Details

This function is mainly a wrapper of the work of others. Please make a reference to ANCOMBC::ancombc2() if you use this function.

Value

The result of ANCOMBC::ancombc2() function

Author(s)

Adrien Taudière

Examples

if (requireNamespace("mia")) {
  data_fungi_mini@tax_table <- phyloseq::tax_table(cbind(
    data_fungi_mini@tax_table,
    "taxon" = taxa_names(data_fungi_mini)
  ))
  res_height <- ancombc_pq(
    data_fungi_mini,
    fact = "Height",
    levels_fact = c("Low", "High"),
    verbose = TRUE
  )

  ggplot(
    res_height$res,
    aes(
      y = reorder(taxon, lfc_HeightHigh),
      x = lfc_HeightHigh,
      color = diff_HeightHigh
    )
  ) +
    geom_vline(xintercept = 0) +
    geom_segment(aes(
      xend = 0, y = reorder(taxon, lfc_HeightHigh),
      yend = reorder(taxon, lfc_HeightHigh)
    ), color = "darkgrey") +
    geom_point()

  res_time <- ancombc_pq(
    data_fungi_mini,
    fact = "Time",
    levels_fact = c("0", "15"),
    tax_level = "Family",
    verbose = TRUE
  )
}

MiscMetabar documentation built on Oct. 8, 2024, 1:07 a.m.