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estimMk<-function(file = NULL, seq = NULL, E, k){
#require(seqinr)
## get the sequences from the given file
if(!is.null(file)){
fasta = read.fasta(file = file)
seq = getSequence(fasta)
attr = getAnnot(fasta)
}
## verify the existence of the sequence
if( !is.list(seq) ){
stop("The parameter \"seq\" should be a list")
}
## get the state space size
S = length(E)
vect.seq<-NULL
## get the number of sequences
nbSeq<-length(seq)
SeqP1 = NULL
Ni = vector(length = S)
## count the number of transitions from i to j in k steps
Nij = matrix(0, nrow = S^k, ncol = S)
for (i in 1:nbSeq){
vect.seq = seq[[i]]
Nij <- Nij + matrix(count(seq = vect.seq, wordsize = k+1, alphabet = E), byrow=TRUE, ncol=S)
## count the number of states i
Ni <- Ni + as.vector(count(seq = vect.seq[1:(length(vect.seq)-k)], wordsize = k, alphabet = E))
## get the state at position 1 for each sequence
SeqP1 = c(SeqP1, vect.seq[1])
}
## Verify the existence of all transitions
if( length(which(Nij == 0)) > 0 ){
warning("missing transitions")
}
## Verify the existence of all states
if( length(which(Ni == 0)) > 0 ){
warning("missing observed states")
}
## Compute the transition matrix
Ptrans<-Nij/(Ni)
Ptrans[which(Ni == 0),] = rep(0, S)
if (nbSeq > 1 ){
if (k == 1){
init = .stationnary.law(Pest = Ptrans) ## !!!Warning if k > 1
## stationnary law
}else{
Nstart = as.vector(count(seq = unlist(seq), wordsize = 1, alphabet = E)) ## initial law for k > 1 ???
init = Nstart/sum(Nstart)
}
}else{
if (k == 1){
init = .stationnary.law(Pest = Ptrans) ## !!! Warning if k > 1
## stationnary law
}else{
Nstart = as.vector(count(seq = unlist(seq), wordsize = 1, alphabet = E)) ## initial law for k > 1 ???
init = Nstart/sum(Nstart)
}
}
return(list(init = init, Ptrans = Ptrans))
}
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