ddpcr: Analysis and Visualization of Droplet Digital PCR in R and on the Web
Version 1.6

An interface to explore, analyze, and visualize droplet digital PCR (ddPCR) data in R. This is the first non-proprietary software for analyzing two-channel ddPCR data. An interactive tool was also created and is available online to facilitate this analysis for anyone who is not comfortable with using R.

AuthorDean Attali [aut, cre]
Date of publication2016-11-12 00:09:57
MaintainerDean Attali <daattali@gmail.com>
LicenseMIT + file LICENSE
Version1.6
URL https://github.com/daattali/ddpcr
Package repositoryView on CRAN
InstallationInstall the latest version of this package by entering the following in R:
install.packages("ddpcr")

Getting started

README.md
Algorithms in ddpcr analysis"
Extending ddpcr by adding new plate types"
Implementation technical details"
Package ddpcr"

Popular man pages

calc_negative_freq_simple: Calculate negative frequency based on number of drops
calculate_concentration: Calculate template concentration
calculate_concentration_single: Calculate concentration in a single well
cluster: Get cluster ID by name
ddpcr_plate: Plate type: ddPCR plate
grapes-greater-than-grapes: magrittr forward-pipe operator
thresholds: Get/set the thresholds
See all...

All man pages Function index File listing

Man pages

analysis_complete: Is the analysis complete?
analyze: Run analysis on a ddPCR plate
bind_df_ends: Helper function for move_front and move_back
calc_negative_freq_simple: Calculate negative frequency based on number of drops
calculate_concentration: Calculate template concentration
calculate_concentration_single: Calculate concentration in a single well
calculate_negative_freqs: Calculate negative frequencies in whole plate
calculate_neg_freq_single: Calculate negative frequency of a single well
capitalize: Capitalize the first letter of a string
cat0: Concatenate strings with no space between them
check_step: Ensure the plate's status is at the right step
classify_droplets: Analysis step: Classify droplets
classify_droplets.pnpp_experiment: Analysis step: Classify droplets
classify_droplets_single: Classify droplets in a well
classify_droplets_single.pnpp_experiment: Classify droplets in a well
classify_thresholds: Analysis step: Classify droplets
cluster: Get cluster ID by name
cluster_name: Get cluster name by ID
clusters: Potential droplet clusters for a plate type
col_to_num: Convert a plate column to a number
custom_thresholds: Plate type: custom thresholds
ddpcr: Analysis and visualization of Droplet Digital PCR data.
ddpcr_plate: Plate type: ddPCR plate
define_clusters: Define droplet clusters
define_clusters.custom_thresholds: Define droplet clusters for custom thresholds plates
define_clusters.ddpcr_plate: Define droplet clusters for default plates
define_clusters.pnpp_experiment: Define droplet clusters for PNPP experiments
define_params: Define plate type parameters
define_params.custom_thresholds: Define plate type parameters for custom thresholds plates
define_params.ddpcr_plate: Define plate type parameters for default plates
define_params.fam_positive_pnpp: Define plate type parameters for FAM-positive PNPP
define_params.hex_positive_pnpp: Define plate type parameters for HEX-positive PNPP
define_params.pnpp_experiment: Define plate type parameters for PNPP experiments
define_params.wildtype_mutant_pnpp: Define plate type parameters for wildtype/mutant PNPP
define_steps: Define analysis steps
define_steps.custom_thresholds: Define analysis steps for custom thresholds plates
define_steps.ddpcr_plate: Define analysis steps for default plates
define_steps.pnpp_experiment: Define analysis steps for PNPP experiments
diff.point2d: Euclidean distance between two points
err_msg: Show an error message
fam_positive_pnpp: Plate type: FAM-positive PNPP
get_col: Get column from well ID
get_empty_cutoff: Get the cutoff for empty droplets in a well
get_empty_cutoff.ddpcr_plate: Get the cutoff for empty droplets in a well
get_empty_cutoff.pnpp_experiment: Get the cutoff for empty droplets in a well
get_filled_border: Get border of filled droplets in PNPP experiment
get_filled_drops: Get filled droplets in PNPP experiment
get_outlier_cutoff: Get the cutoff for outliers
get_outlier_cutoff.ddpcr_plate: Get the cutoff for outliers
get_row: Get row from well ID
get_single_well: Get droplet data from a well
get_wells_btwn: Get all wells between two wells (assume a rectangle layout)
grapes-btwn-grapes: Determine if a numeric value is within a range
grapes-greater-than-grapes: magrittr forward-pipe operator
has_signif_negative_cluster: Does a well have a statistically significant number of...
has_step: Does a ddPCR plate have a step with this name?
hex_positive_pnpp: Plate type: HEX-positive PNPP
is_dir: Determine if a given path is a valid directory
is_dirty: Is the plate object dirty (ie has changed since the analysis...
is_empty_plate: Is a plate empty?
is_file: Determine if a given path is a valid file
is_range: Is the given parameter a range?
is_well_success: Determine if a well had a successful ddPCR run
is_well_success.ddpcr_plate: Determine if a well had a successful ddPCR run
is_well_success.pnpp_experiment: Determine if a well had a successful ddPCR run
launch: Run the interactive analysis tool (Shiny app) in a web...
load_plate: Load a previously saved ddPCR plate
local_maxima: Get the indices of the local maxima in a list of numbers
local_minima: Get the indices of the local minima in a list of numbers
lol_to_df: Convert a list of lists returned from vapply to a dataframe
mark_clusters: Mark the clusters of droplets only in certain wells to their...
merge_dfs_overwrite_col: Overwrite a column in a data.frame based on a matching column...
meta_var_name: Name of variable in PNPP experiment metadata
move_back: Move columns to the back of a data.frame
move_front: Move columns to the front of a data.frame
msg: Write a message to the user if the 'ddpcr.verbose' option is...
name: Plate name
named_vec_to_df: Convert a named vector returned from vapply to a dataframe
new_plate: Create a new ddPCR plate
next_step: Run the next step in an analysis
normalize_to_rds: Normalize a file name to .rds suffix
num_to_col: Convert a number to plate column
num_to_row: Convert a number to plate row
other_dim: Given an axis (X or Y), return the other
params: Plate parameters
parent_plate_type: Parent plate type
parent_plate_type.ddpcr_plate: Parent plate type of default plates
parent_plate_type.default: Parent plate type of any plate
parent_plate_type.fam_positive_pnpp: Parent plate type of FAM-positive PNPP
parent_plate_type.hex_positive_pnpp: Parent plate type of HEX-positive PNPP
parent_plate_type.wildtype_mutant_pnpp: Parent plate type of wildtype/mutant PNPP
plate_data: Plate data (droplets data)
plate_data-set: Overwrite the plate data
plate_meta: Plate metadata
plate_meta-set: Overwrite the plate metadata
plate_types: Supported plate types
plot.custom_thresholds: Plot a ddPCR plate of type custom thresholds
plot.ddpcr_plate: Plot a ddPCR plate
plot.pnpp_experiment: Plot a ddPCR plate of type PNPP experiment
plot.wildtype_mutant_pnpp: Plot a ddPCR plate of type wildtype/mutant PNPP
pnpp_experiment: Plate type: PNPP experiment
point2d: Representation of a 2D point
positive_dim: Positive dimension in a PNPP experiment
positive_dim_var: Name of dye in positive dimension in PNPP experiment
positive_name: Name identifier for positive and negative droplets
print.ddpcr_plate: Print info about a ddPCR plate
quiet: Suppress all output from an expression. Works cross-platform.
range_list_to_vec: Convert a list of ranges to a vector of its individual...
range_to_endpoints: Extract the two endpoints of a range
range_to_seq: Convert a range to a vector of all elements between the...
reclassify_droplets: Analysis step: Reclassify droplets
reclassify_droplets.pnpp_experiment: Analysis step: Reclassify droplets
reclassify_droplets_single: Reclassify droplets in a well
reclassify_droplets_single.pnpp_experiment: Reclassify droplets in a well
remove_empty: Analysis step: Remove empty droplets
remove_empty.ddpcr_plate: Analysis step: Remove empty droplets
remove_empty.pnpp_experiment: Analysis step: Remove empty droplets
remove_failures: Analysis step: Remove failed wells
remove_failures.ddpcr_plate: Analysis step: Remove failed wells
remove_outliers: Analysis Step: Remove outlier droplets
remove_outliers.ddpcr_plate: Analysis Step: Remove outlier droplets
reset: Reset a plate
row_to_num: Convert a plate row to a number
sample_data: Get sample data
save_plate: Save a ddPCR plate
set_default_params: Reset plate parameters to their defaults
status: Plate status
step: Get step ID by step name
step_begin: Inform the user that an analysis step is starting
step_end: Inform the user that an analysis step finished
step_name: Get step name by ID
steps: Analysis steps of a ddPCR plate
subset.ddpcr_plate: Subsetting a ddPCR plate
thresholds: Get/set the thresholds
type: Plate type
unanalyzed_clusters: Get unanalyzed cluseter IDs
variable_dim: Variable dimension in a PNPP experiment
variable_dim_var: Name of dye in variable dimension in PNPP experiment
warn_msg: Show a warning message
WELL_ID_REGEX: regex for a well ID
well_info: Get metadata info of a well
wells_mutant: Get mutant wells
wells_negative: Get negative wells
wells_positive: Get positive wells
wells_success: Get successful/failed wells
wells_used: Get wells used in a ddPCR plate
wells_wildtype: Get wildtype wells
wildtype_mutant_pnpp: Plate type: wildtype/mutant PNPP
x_threshold: Get/set the X threshold
x_var: Get/set the X/Y variable (dye name)
y_threshold: Get/set the Y threshold

Functions

WELL_ID_REGEX Man page
\%>\% Man page
\%btwn\% Man page
analysis_complete Man page Source code
analyze Man page Source code
arrange_meta Source code
bind_df_ends Man page Source code
calc_negative_freq_simple Man page Source code
calculate_concentration Man page Source code
calculate_concentration_single Man page Source code
calculate_neg_freq_single Man page Source code
calculate_negative_freqs Man page Source code
capitalize Man page Source code
cat0 Man page Source code
check_step Man page Source code
classify_droplets Man page Source code
classify_droplets.pnpp_experiment Man page Source code
classify_droplets_single Man page Source code
classify_droplets_single.pnpp_experiment Man page Source code
classify_thresholds Man page Source code
cluster Man page Source code
cluster_name Man page Source code
clusters Man page Source code
col_to_num Man page Source code
custom_thresholds Man page
ddpcr Man page
ddpcr-package Man page
ddpcr_plate Man page
define_clusters Man page Source code
define_clusters.custom_thresholds Man page Source code
define_clusters.ddpcr_plate Man page Source code
define_clusters.pnpp_experiment Man page Source code
define_params Man page Source code
define_params.custom_thresholds Man page Source code
define_params.ddpcr_plate Man page Source code
define_params.fam_positive_pnpp Man page Source code
define_params.hex_positive_pnpp Man page Source code
define_params.pnpp_experiment Man page Source code
define_params.wildtype_mutant_pnpp Man page Source code
define_steps Man page Source code
define_steps.custom_thresholds Man page Source code
define_steps.ddpcr_plate Man page Source code
define_steps.pnpp_experiment Man page Source code
diff.point2d Man page Source code
empty_plate Source code
err_msg Man page Source code
fam_positive_pnpp Man page
find_data_files Source code
find_meta_file Source code
format.point2d Source code
get_col Man page Source code
get_consensus_name_from_data_files Source code
get_empty_cutoff Man page Source code
get_empty_cutoff.ddpcr_plate Man page Source code
get_empty_cutoff.pnpp_experiment Man page Source code
get_filled_border Man page Source code
get_filled_drops Man page Source code
get_name_from_data_file Source code
get_outlier_cutoff Man page Source code
get_outlier_cutoff.ddpcr_plate Man page Source code
get_row Man page Source code
get_single_well Man page Source code
get_well_from_data_file Source code
get_wells_btwn Man page Source code
has_signif_negative_cluster Man page Source code
has_step Man page Source code
hex_positive_pnpp Man page
init_data Source code
init_meta Source code
init_plate Source code
is_dir Man page Source code
is_dirty Man page Source code
is_empty_plate Man page Source code
is_file Man page Source code
is_range Man page Source code
is_well_success Man page Source code
is_well_success.ddpcr_plate Man page Source code
is_well_success.pnpp_experiment Man page Source code
launch Man page Source code
load_plate Man page Source code
local_maxima Man page Source code
local_minima Man page Source code
lol_to_df Man page Source code
mark_clusters Man page Source code
merge_dfs_overwrite_col Man page Source code
meta_var_name Man page Source code
move_back Man page Source code
move_front Man page Source code
msg Man page Source code
name Man page Source code
name<- Man page
named_vec_to_df Man page Source code
negative_name Man page Source code
negative_name<- Man page
nevercalled Source code
new_plate Man page Source code
next_step Man page Source code
normalize_to_rds Man page Source code
num_to_col Man page Source code
num_to_row Man page Source code
other_dim Man page Source code
params Man page Source code
params<- Man page
parent_plate_type Man page Source code
parent_plate_type.ddpcr_plate Man page Source code
parent_plate_type.default Man page Source code
parent_plate_type.fam_positive_pnpp Man page Source code
parent_plate_type.hex_positive_pnpp Man page Source code
parent_plate_type.wildtype_mutant_pnpp Man page Source code
pkg_globals_store Source code
plate_data Man page Source code
plate_data<- Man page
plate_meta Man page Source code
plate_meta<- Man page
plate_types Man page
plot.custom_thresholds Man page Source code
plot.ddpcr_plate Man page Source code
plot.pnpp_experiment Man page Source code
plot.wildtype_mutant_pnpp Man page Source code
pnpp_experiment Man page
point2d Man page Source code
positive_dim Man page Source code
positive_dim<- Man page
positive_dim_var Man page Source code
positive_name Man page Source code
positive_name<- Man page
print.ddpcr_plate Man page Source code
print.point2d Source code
quiet Man page Source code
range_list_to_vec Man page Source code
range_to_endpoints Man page Source code
range_to_seq Man page Source code
read_dir Source code
read_files Source code
read_plate Source code
reclassify_droplets Man page Source code
reclassify_droplets.pnpp_experiment Man page Source code
reclassify_droplets_single Man page Source code
reclassify_droplets_single.pnpp_experiment Man page Source code
remove_empty Man page Source code
remove_empty.ddpcr_plate Man page Source code
remove_empty.pnpp_experiment Man page Source code
remove_failures Man page Source code
remove_failures.ddpcr_plate Man page Source code
remove_outliers Man page Source code
remove_outliers.ddpcr_plate Man page Source code
reset Man page Source code
row_to_num Man page Source code
sample_data Man page
sample_data_dir Man page Source code
sample_data_file Man page Source code
sample_plate Man page Source code
sample_results_file Man page Source code
save_plate Man page Source code
set_default_clusters Source code
set_default_params Man page Source code
set_default_steps Source code
set_plate_type Source code
set_thresholds Man page Source code
setup_new_plate Source code
setup_plate Source code
status Man page Source code
status<- Man page
step Man page Source code
step_begin Man page Source code
step_end Man page Source code
step_name Man page Source code
steps Man page Source code
subset.ddpcr_plate Man page Source code
thresholds Man page Source code
thresholds<- Man page
type Man page Source code
unanalyzed_clusters Man page Source code
variable_dim Man page Source code
variable_dim<- Man page
variable_dim_var Man page Source code
warn_msg Man page Source code
well_info Man page Source code
wells_failed Man page Source code
wells_mutant Man page Source code
wells_negative Man page Source code
wells_positive Man page Source code
wells_success Man page Source code
wells_used Man page Source code
wells_wildtype Man page Source code
wildtype_mutant_pnpp Man page
x_threshold Man page Source code
x_threshold<- Man page
x_var Man page Source code
x_var<- Man page
y_threshold Man page Source code
y_threshold<- Man page
y_var Man page Source code
y_var<- Man page

Files

inst
inst/vignettes-supp
inst/vignettes-supp/pnpp-simple-result.png
inst/vignettes-supp/outliers.png
inst/vignettes-supp/algorithms-imgs.R
inst/vignettes-supp/gating.png
inst/vignettes-supp/ddpcr-example.png
inst/vignettes-supp/vignette_data_B06_Amplitude.csv
inst/vignettes-supp/supported-exp-types.png
inst/vignettes-supp/empty.png
inst/vignettes-supp/ex-subset.png
inst/shiny
inst/shiny/ui.R
inst/shiny/server.R
inst/shiny/www
inst/shiny/www/hexpositive.png
inst/shiny/www/ddpcr.js
inst/shiny/www/favicon.ico
inst/shiny/www/ajax-loader.gif
inst/shiny/www/fampositive.png
inst/shiny/www/ajax-loader-bar.gif
inst/shiny/www/style.css
inst/shiny/ui
inst/shiny/ui/tab-dataset.R
inst/shiny/ui/header.R
inst/shiny/ui/tab-about.R
inst/shiny/ui/helpers.R
inst/shiny/ui/tab-results.R
inst/shiny/ui/tab-settings.R
inst/shiny/ui/tab-analyze.R
inst/shiny/server
inst/shiny/server/tab-dataset.R
inst/shiny/server/helpers.R
inst/shiny/server/tab-results.R
inst/shiny/server/tab-settings.R
inst/shiny/server/tab-analyze.R
inst/shiny/text
inst/shiny/text/about.md
inst/sample_data
inst/sample_data/large
inst/sample_data/large/large.rds
inst/sample_data/read_complex
inst/sample_data/read_complex/metadata.csv
inst/sample_data/read_complex/test_A01_Amplitude.csv
inst/sample_data/read_complex/test_B02_Amplitude.csv
inst/sample_data/read_complex/diffname_C03_Amplitude.csv
inst/sample_data/read_complex/expected_data.csv
inst/sample_data/small
inst/sample_data/small/A05_filled.csv
inst/sample_data/small/small.rds
inst/sample_data/small/small.csv
inst/sample_data/small/small_A01_Amplitude.csv
inst/sample_data/small/small_F05_Amplitude.csv
inst/sample_data/small/small_C05_Amplitude.csv
inst/sample_data/small/analyzed.rds
inst/sample_data/small/small_A05_Amplitude.csv
inst/sample_data/small/analyzed_pnpp.rds
inst/sample_data/small/small_C01_Amplitude.csv
inst/sample_data/read_simple
inst/sample_data/read_simple/test_A01_Amplitude.csv
inst/sample_data/read_simple/test_B02_Amplitude.csv
inst/sample_data/read_simple/test.csv
inst/sample_data/read_simple/expected_data.csv
inst/sample_data/empty_plate.rds
inst/doc
inst/doc/overview.R
inst/doc/technical_details.Rmd
inst/doc/technical_details.html
inst/doc/algorithm.Rmd
inst/doc/algorithm.R
inst/doc/extend.html
inst/doc/extend.Rmd
inst/doc/overview.html
inst/doc/overview.Rmd
inst/doc/extend.R
inst/doc/technical_details.R
inst/doc/algorithm.html
tests
tests/testthat.R
tests/testthat
tests/testthat/test-utils.R
tests/testthat/test-merge_dfs_overwrite_col.R
tests/testthat/test-empty.R
tests/testthat/test-plate-attribs.R
tests/testthat/test-pnpp_experiment-calculate_neg_freq.R
tests/testthat/test-subset.R
tests/testthat/test-failures.R
tests/testthat/test-plate.R
tests/testthat/test-outliers.R
tests/testthat/test-pnpp_experiment-filled.R
tests/testthat/test-save_load.R
tests/testthat/test-pnpp_experiment.R
tests/testthat/test-custom_thresholds.R
tests/testthat/test-read.R
NAMESPACE
NEWS.md
R
R/type-custom_thresholds.R
R/type-wildtype_mutant_pnpp.R
R/utils.R
R/type-hex_positive_pnpp.R
R/empty.R
R/aaa.R
R/outliers.R
R/pnpp_experiment-failures.R
R/subset.R
R/plate-attribs.R
R/plate.R
R/pnpp_experiment-empty.R
R/plot.R
R/pipe.R
R/launch.R
R/sample_data.R
R/plate-utils.R
R/concentration.R
R/plate-extend.R
R/ddpcr.R
R/pnpp_experiment-reclassify.R
R/type-fam_positive_pnpp.R
R/pnpp_experiment-calculate_neg_freq.R
R/save_load.R
R/type-ddpcr_plate.R
R/plate-print.R
R/type-pnpp_experiment.R
R/read.R
R/pnpp_experiment-classify.R
R/ignoreme.R
R/pnpp_experiment-plot.R
R/failures.R
R/zzz.R
R/pnpp_experiment-filled.R
vignettes
vignettes/technical_details.Rmd
vignettes/algorithm.Rmd
vignettes/extend.Rmd
vignettes/overview.Rmd
README.md
MD5
build
build/vignette.rds
DESCRIPTION
man
man/launch.Rd
man/define_params.hex_positive_pnpp.Rd
man/parent_plate_type.hex_positive_pnpp.Rd
man/step_end.Rd
man/parent_plate_type.default.Rd
man/grapes-greater-than-grapes.Rd
man/normalize_to_rds.Rd
man/type.Rd
man/classify_droplets.pnpp_experiment.Rd
man/new_plate.Rd
man/get_empty_cutoff.ddpcr_plate.Rd
man/classify_thresholds.Rd
man/params.Rd
man/y_threshold.Rd
man/sample_data.Rd
man/local_minima.Rd
man/calculate_concentration_single.Rd
man/mark_clusters.Rd
man/parent_plate_type.ddpcr_plate.Rd
man/wells_used.Rd
man/unanalyzed_clusters.Rd
man/positive_dim.Rd
man/status.Rd
man/plot.ddpcr_plate.Rd
man/define_clusters.Rd
man/calc_negative_freq_simple.Rd
man/range_to_seq.Rd
man/define_params.custom_thresholds.Rd
man/define_clusters.ddpcr_plate.Rd
man/define_params.Rd
man/wells_mutant.Rd
man/classify_droplets_single.Rd
man/capitalize.Rd
man/positive_name.Rd
man/ddpcr.Rd
man/num_to_row.Rd
man/move_front.Rd
man/ddpcr_plate.Rd
man/save_plate.Rd
man/wells_positive.Rd
man/named_vec_to_df.Rd
man/reclassify_droplets_single.Rd
man/define_steps.pnpp_experiment.Rd
man/classify_droplets.Rd
man/subset.ddpcr_plate.Rd
man/classify_droplets_single.pnpp_experiment.Rd
man/define_steps.Rd
man/load_plate.Rd
man/cluster.Rd
man/get_filled_drops.Rd
man/next_step.Rd
man/get_filled_border.Rd
man/is_dirty.Rd
man/pnpp_experiment.Rd
man/quiet.Rd
man/get_outlier_cutoff.ddpcr_plate.Rd
man/WELL_ID_REGEX.Rd
man/warn_msg.Rd
man/calculate_negative_freqs.Rd
man/move_back.Rd
man/is_file.Rd
man/analyze.Rd
man/lol_to_df.Rd
man/is_well_success.pnpp_experiment.Rd
man/get_empty_cutoff.pnpp_experiment.Rd
man/remove_failures.Rd
man/range_list_to_vec.Rd
man/parent_plate_type.wildtype_mutant_pnpp.Rd
man/positive_dim_var.Rd
man/fam_positive_pnpp.Rd
man/remove_empty.ddpcr_plate.Rd
man/err_msg.Rd
man/remove_outliers.ddpcr_plate.Rd
man/row_to_num.Rd
man/meta_var_name.Rd
man/point2d.Rd
man/step_name.Rd
man/remove_failures.ddpcr_plate.Rd
man/reclassify_droplets.Rd
man/msg.Rd
man/variable_dim.Rd
man/define_params.fam_positive_pnpp.Rd
man/custom_thresholds.Rd
man/parent_plate_type.fam_positive_pnpp.Rd
man/step.Rd
man/define_params.wildtype_mutant_pnpp.Rd
man/wells_wildtype.Rd
man/name.Rd
man/steps.Rd
man/plate_data-set.Rd
man/thresholds.Rd
man/define_clusters.custom_thresholds.Rd
man/merge_dfs_overwrite_col.Rd
man/get_wells_btwn.Rd
man/plot.custom_thresholds.Rd
man/bind_df_ends.Rd
man/has_step.Rd
man/clusters.Rd
man/cluster_name.Rd
man/is_well_success.ddpcr_plate.Rd
man/parent_plate_type.Rd
man/get_row.Rd
man/get_single_well.Rd
man/x_threshold.Rd
man/is_empty_plate.Rd
man/plate_types.Rd
man/plot.wildtype_mutant_pnpp.Rd
man/define_params.pnpp_experiment.Rd
man/reclassify_droplets_single.pnpp_experiment.Rd
man/plate_meta-set.Rd
man/get_empty_cutoff.Rd
man/wells_negative.Rd
man/well_info.Rd
man/x_var.Rd
man/diff.point2d.Rd
man/local_maxima.Rd
man/plate_meta.Rd
man/define_steps.custom_thresholds.Rd
man/plot.pnpp_experiment.Rd
man/col_to_num.Rd
man/step_begin.Rd
man/set_default_params.Rd
man/get_col.Rd
man/wells_success.Rd
man/remove_outliers.Rd
man/calculate_neg_freq_single.Rd
man/num_to_col.Rd
man/print.ddpcr_plate.Rd
man/define_clusters.pnpp_experiment.Rd
man/remove_empty.pnpp_experiment.Rd
man/plate_data.Rd
man/is_range.Rd
man/is_dir.Rd
man/range_to_endpoints.Rd
man/has_signif_negative_cluster.Rd
man/grapes-btwn-grapes.Rd
man/is_well_success.Rd
man/get_outlier_cutoff.Rd
man/calculate_concentration.Rd
man/cat0.Rd
man/wildtype_mutant_pnpp.Rd
man/remove_empty.Rd
man/define_steps.ddpcr_plate.Rd
man/reclassify_droplets.pnpp_experiment.Rd
man/analysis_complete.Rd
man/check_step.Rd
man/define_params.ddpcr_plate.Rd
man/variable_dim_var.Rd
man/other_dim.Rd
man/reset.Rd
man/hex_positive_pnpp.Rd
LICENSE
ddpcr documentation built on May 19, 2017, 11:23 p.m.

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