classify_droplets: Analysis step: Classify droplets

Description Usage Arguments Details Value Note See Also

View source: R/pnpp_experiment-classify.R

Description

The main analysis step for ddPCR plates of type pnpp_experiment. Classify each droplet as either rain, ++, or +-. Also calculate the frequency of negative droplets, and attempt to detemine if each well has a statistically significant number of such droplets.

See the README for more information about the algorithm used.

Usage

1

Arguments

plate

A ddPCR plate.

Details

This function is recommended to be run as part of an analysis pipeline (ie. within the analyze function) rather than being called directly.

Value

A ddPCR plate with all droplets assigned to a cluster. The plate's metadata will have several new variables.

Note

This is an S3 generic, which means that different ddPCR plate types can implement this function differently. See the README for more information on how to implement custom ddPCR plate types.

See Also

analyze
classify_droplets_single
mark_clusters
has_signif_negative_cluster


ddpcr documentation built on July 8, 2020, 6:49 p.m.