classify_droplets: Analysis step: Classify droplets
In ddpcr: Analysis and Visualization of Droplet Digital PCR in R and on the Web
View source: R/pnpp_experiment-classify.R
classify_droplets R Documentation
Analysis step: Classify droplets
Description
The main analysis step for ddPCR plates of type pnpp_experiment.
Classify each droplet as either rain, ++, or +-. Also calculate the frequency
of negative droplets, and attempt to detemine if each well has a statistically
significant number of such droplets.
See the README for
more information about the algorithm used.
Usage
classify_droplets(plate)
Arguments
plate
A ddPCR plate.
Details
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze function) rather than being called
directly.
Value
A ddPCR plate with all droplets assigned to a cluster. The plate's
metadata will have several new variables.
Note
This is an S3 generic, which means that different ddPCR plate types can
implement this function differently.
See the README for
more information on how to implement custom ddPCR plate types.
See Also
analyze
classify_droplets_single
mark_clusters
has_signif_negative_cluster
ddpcr documentation built on Aug. 21, 2023, 1:07 a.m.
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View source: R/pnpp_experiment-classify.R
| classify_droplets | R Documentation |
Analysis step: Classify droplets
Description
The main analysis step for ddPCR plates of type pnpp_experiment.
Classify each droplet as either rain, ++, or +-. Also calculate the frequency
of negative droplets, and attempt to detemine if each well has a statistically
significant number of such droplets.
See the README for
more information about the algorithm used.
Usage
classify_droplets(plate)
Arguments
plate |
A ddPCR plate. |
Details
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze function) rather than being called
directly.
Value
A ddPCR plate with all droplets assigned to a cluster. The plate's metadata will have several new variables.
Note
This is an S3 generic, which means that different ddPCR plate types can implement this function differently. See the README for more information on how to implement custom ddPCR plate types.
See Also
analyze
classify_droplets_single
mark_clusters
has_signif_negative_cluster
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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