calculate_concentration: Calculate template concentration
In ddpcr: Analysis and Visualization of Droplet Digital PCR in R and on the Web
View source: R/concentration.R
calculate_concentration R Documentation
Calculate template concentration
Description
Calculate template concentration in each wells in a plate using the same formula that
QuantaSoft uses. The concentration information is added to the plate's metadata.
Usage
calculate_concentration(plate)
Details
The concentration in a well as number of copies of template per microlitre
of sample, and uses the following equation:
(-log(drops_empty / drops_total) * drops_total) / (droplet_volume * drops_total)
Value
A ddPCR plate with the metadata containing a new 'concentration' variable.
ddpcr documentation built on Aug. 21, 2023, 1:07 a.m.
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View source: R/concentration.R
| calculate_concentration | R Documentation |
Calculate template concentration
Description
Calculate template concentration in each wells in a plate using the same formula that QuantaSoft uses. The concentration information is added to the plate's metadata.
Usage
calculate_concentration(plate)
Details
The concentration in a well as number of copies of template per microlitre of sample, and uses the following equation:
(-log(drops_empty / drops_total) * drops_total) / (droplet_volume * drops_total)
Value
A ddPCR plate with the metadata containing a new 'concentration' variable.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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