Description Usage Arguments Details Value References Examples
View source: R/loq_derivatives.R
Estimates the limits of quantification based on the second order derivative of the functions.
1 | loq_derivatives(x, subset.list = NULL, ...)
|
x |
a |
subset.list |
list of analytes to estimate.
Default |
... |
further arguments to be passed to |
The limits of quantification are based on the
maximum and minimum points of the second order derivative of the function.
The solution is based on the specific expressions of the derivatives.
The uniroot.all
function is used
in order to find the global maximum and minimum.
Object of class loq
.
Ritz C and Spiess AN (2008). qpcR: an R package for sigmoidal model selection in q uantitative real-time polymerase chain reaction analysis. Bioinformatics 24, 1549-51.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 | # Load data and estimate models
data(ecdata)
data(mfidata)
dat <- mfidata[mfidata$plate=="plate_1" & mfidata$analyte=="FGF",]
sdf <- data_selection(dat, ecdata)[[1]]
igmodels <- scluminex("plate_1",sdf$standard, sdf$background,
lfct=c("SSl4", "SSl5"),
bkg="ignore",
fmfi="mfi",
verbose=FALSE)
loq_derivatives(igmodels)
|
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