ifcb_merge_manual: Merge IFCB Manual Classification Data

In iRfcb: Tools for Managing Imaging FlowCytobot (IFCB) Data

Merge IFCB Manual Classification Data

Description

This function merges two sets of manual classification data by combining and aligning class labels from a base set and an additional set of classifications. The merged .mat data can be used with the code in the ifcb-analysis repository (Sosik and Olson 2007).

Usage

ifcb_merge_manual(
  class2use_file_base,
  class2use_file_additions,
  class2use_file_output = NULL,
  manual_folder_base,
  manual_folder_additions,
  manual_folder_output,
  do_compression = TRUE,
  temp_index_offset = 50000,
  skip_class = NULL,
  quiet = FALSE
)

Arguments

class2use_file_base	Character. Path to the class2use file of the base manual classifications. The base set contains the original manual classifications list that form the foundation for merging.
class2use_file_additions	Character. Path to the class2use file of the additions manual classifications. The additions set contains additional classifications that need to be merged with the base set. Class labels from the class2use_file_additions that are not already included in the class2use_file_base will be added to generate the class2use_file_output.
class2use_file_output	Character. Path where the merged class2use file will be saved. If NULL, the merged file will be stored in the same directory as class2use_file_base. Default is NULL.
manual_folder_base	Character. Path to the folder containing the base set of manual classification .mat files.
manual_folder_additions	Character. Path to the folder containing the additions set of manual classification .mat files.
manual_folder_output	Character. Path to the output folder where the merged classification files will be stored.
do_compression	A logical value indicating whether to compress the .mat file. Defaults to TRUE.
temp_index_offset	Numeric. A large integer used to generate temporary indices during the merge process. Default is 50000.
skip_class	Character. A vector of class names to skip from the class2use_file_additions during the merge process. Default is NULL.
quiet	Logical. If TRUE, suppresses output messages. Default is FALSE.

Details

Python must be installed to use this function. The required python packages can be installed in a virtual environment using ifcb_py_install().

The base set consists of the original classifications that are used as a reference for the merging process. The additions set contains the additional classifications that need to be merged with the base set. When merging, unique class names from the additions set that are not present in the base set are appended.

The function works by aligning the class labels from the additions set with those in the base set, handling conflicts by using a temporary index system. It copies .mat files from both the base and additions folders into the output folder, while adjusting indices and and class names for the additions.

Note that the maximum limit for uint16 is 65,535, so ensure that temp_index_offset remains below this value.

Value

No return value. Outputs the combined class2use file in the same folder as class2use_file_base is located or at a user-specified location, and merged .mat files into the output folder.

References

Sosik, H. M. and Olson, R. J. (2007), Automated taxonomic classification of phytoplankton sampled with imaging-in-flow cytometry. Limnol. Oceanogr: Methods 5, 204–216.

See Also

ifcb_py_install https://github.com/hsosik/ifcb-analysis

Examples

## Not run: 
ifcb_merge_manual("path/to/class2use_base.mat", "path/to/class2use_additions.mat",
                  "path/to/class2use_combined.mat", "path/to/manual/base_folder",
                  "path/to/manual/additions_folder", "path/to/manual/output_folder",
                  do_compression = TRUE, temp_index_offset = 50000, quiet = FALSE)

## End(Not run)

iRfcb documentation built on April 16, 2025, 1:09 a.m.