README.md
In rgenie: Analysis of GenIE Experiments

rgenie

rgenie is an R package to analyze the sequencing output from a set of GenIE experimental replicates.

If you don't have devtools yet, first install devtools:

install.packages("devtools")

Then install rgenie using devtools:

devtools::install_github("jeremy37/rgenie")

GenIE (genome-editing interrogation of enhancers) is an experimental method to evaluate the effects of individual SNPs on gene transcription.

Briefly, CRISPR-Cas9 is targeted near a SNP of interest, and typically an oligonucleotide for homology-directed repair (HDR) will be included. With the editing done in a pool of cells, the single nucleotide change can be achieved in a fraction of cell chromosomes, and the remaining chromosomes will either be wild-type or will have deletions in the region. You then extract both RNA and DNA from the pool of cells, and do multiple replicates to amplify an amplicon from each of these (genomic DNA and cDNA). rgenie can help to visualize the experiment and compute statistics for the effects of different alleles on gene transcription.

The plot below shows the set of alleles measured in a set of gDNA and cDNA replicates.

The Introductory vignette shows how to download example data and run an analysis.

The rgenie in depth vignette provides more details on the parameters for some methods and how to interpret results.

Cooper SE, Schwartzentruber J, Bello E, Coomber EL, Bassett AR. Screening for functional transcriptional and splicing regulatory variants with GenIE. Not yet published.