Description Usage Arguments Value Note See Also Examples
This is an enrichmentMatrix
function for ScoreMatrix
objects,
that enables to normalize ChIP-seq signals with respect to IgG or input DNA control.
1 | \S4method{enrichmentMatrix}{ScoreMatrix,ScoreMatrix}(IP, control)
|
IP |
|
control |
|
ScoreMatrix
object
The function computes an enrichment of IP over control as follow: Suppose both IP and control are ScoreMatrix objects that have same dimensions. Then, the enrichment is calculated usign a formula: log2((IP + 1) / (control + 1)).
1 2 3 4 5 6 7 8 9 10 11 12 13 |
#load IP and control BAM files and create ScoreMatrix objects
library('genomationData')
bam.file_IP <- system.file("extdata",
"wgEncodeBroadHistoneH1hescSuz12051317AlnRep1.chr21.bam", package = "genomationData")
bam.file_c <- system.file("extdata",
"wgEncodeBroadHistoneH1hescCtcfStdAlnRep1.chr21.bam", package = "genomationData")
data(promoters)
IP <- ScoreMatrix(target = bam.file_IP, windows = promoters, type = 'bam')
control <- ScoreMatrix(target = bam.file_c, windows = promoters, type = 'bam')
# compute an enrichment of IP over control
enrichmentMatrix(IP, control)
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