tr2g_EnsDb: Get transcript and gene info from EnsDb objects
In BUStools/BUSpaRse: kallisto | bustools R utilities

tr2g_EnsDb

R Documentation

Get transcript and gene info from EnsDb objects

Description

Bioconductor provides Ensembl genome annotation in AnnotationHub; older versions of Ensembl annotation can be obtained from packages like EnsDb.Hsapiens.v86. This is an alternative to querying Ensembl with biomart; Ensembl's server seems to be less stable than that of Bioconductor. However, more information and species are available on Ensembl biomart than on AnnotationHub.

Usage

tr2g_EnsDb(
  ensdb,
  Genome = NULL,
  get_transcriptome = TRUE,
  out_path = ".",
  write_tr2g = TRUE,
  other_attrs = NULL,
  use_gene_name = TRUE,
  use_transcript_version = TRUE,
  use_gene_version = TRUE,
  transcript_biotype_col = "TXBIOTYPE",
  gene_biotype_col = "GENEBIOTYPE",
  transcript_biotype_use = "all",
  gene_biotype_use = "all",
  chrs_only = TRUE,
  compress_fa = FALSE,
  overwrite = FALSE
)

Arguments

`ensdb`	Ann `EnsDb` object, such as from `AnnotationHub` or `EnsDb.Hsapiens.v86`.
`Genome`	Either a `BSgenome` or a `XStringSet` object of genomic sequences, where the intronic sequences will be extracted from. Use `genomeStyles` to check which styles are supported for your organism of interest; supported styles can be interconverted. If the style in your genome or annotation is not supported, then the style of chromosome names in the genome and annotation should be manually set to be consistent.
`get_transcriptome`	Logical, whether to extract transcriptome from genome with the GTF file. If filtering biotypes or chromosomes, the filtered `GRanges` will be used to extract transcriptome.
`out_path`	Directory to save the outputs written to disk. If this directory does not exist, then it will be created. Defaults to the current working directory.
`write_tr2g`	Logical, whether to write tr2g to disk. If `TRUE`, then a file `tr2g.tsv` will be written into `out_path`.
`other_attrs`	Character vector. Other attributes to get from the `EnsDb` object, such as gene symbol and position on the genome. Use `columns` to see which attributes are available.
`use_gene_name`	Logical, whether to get gene names.
`use_transcript_version`	Logical, whether to include version number in the Ensembl transcript ID. To decide whether to include transcript version number, check whether version numbers are included in the `transcripts.txt` in the `kallisto` output directory. If that file includes version numbers, then trannscript version numbers must be included here as well. If that file does not include version numbers, then transcript version numbers must not be included here.
`use_gene_version`	Logical, whether to include version number in the Ensembl gene ID. Unlike transcript version number, it's up to you whether to include gene version number.
`transcript_biotype_col`	Character vector of length 1. Tag in `attribute` field corresponding to transcript biotype.
`gene_biotype_col`	Character vector of length 1. Tag in `attribute` field corresponding to gene biotype.
`transcript_biotype_use`	Character, can be "all" or a vector of transcript biotypes to be used. Transcript biotypes aren't entirely the same as gene biotypes. For instance, in Ensembl annotation, `retained_intron` is a transcript biotype, but not a gene biotype. If "cellranger", then a warning will be given. See `data("ensembl_tx_biotypes")` for all available transcript biotypes from Ensembl.
`gene_biotype_use`	Character, can be "all", "cellranger", or a vector of gene biotypes to be used. If "cellranger", then the biotypes used by Cell Ranger's reference are used. See `data("cellranger_biotypes")` for gene biotypes the Cell Ranger reference uses. See `data("ensembl_gene_biotypes")` for all available gene biotypes from Ensembl. Note that gene biotypes and transcript biotypes are not always the same.
`chrs_only`	Logical, whether to include chromosomes only, for GTF and GFF files can contain annotations for scaffolds, which are not incorporated into chromosomes. This will also exclude haplotypes. Defaults to `TRUE`. Only applicable to species found in `genomeStyles()`.
`compress_fa`	Logical, whether to compress the output fasta file. If `TRUE`, then the fasta file will be gzipped.
`overwrite`	Logical, whether to overwrite if files with names of outputs written to disk already exist.

Value

A data frame with at least 2 columns: gene for gene ID, transcript for transcript ID, and optionally gene_name for gene names. If other_attrs has been specified, then those will also be columns in the data frame returned.

Examples

library(EnsDb.Hsapiens.v86)
tr2g_EnsDb(EnsDb.Hsapiens.v86, get_transcriptome = FALSE, write_tr2g = FALSE,
 use_transcript_version = FALSE,
 use_gene_version = FALSE)

BUStools/BUSpaRse documentation built on Aug. 2, 2024, 5:07 a.m.