spillover_compute: Compute Spillover Matrix

In DillonHammill/cytoSuite: Compensation, Gating & Visualisation Toolkit for Analysis of Flow Cytometry Data

Description

spillover_compute uses the method described by Bagwell & Adams 1993 to calculate the fluorescent spillover matrix using a reference universal unstained control and single stain compensation controls.

Usage

spillover_compute(x, ...)

Arguments

x	object of class flowSet or GatingSet.
...	additional method-specific arguments for spillover_compute.

Details

spillover_compute begins by the user selecting which fluorescent channel is associated with each control from a dropdown menu. Following channel selection, spillover_compute runs through each control and plots the density distribution of the unstained control in red and the compensation control in blue. Users can then gate the positive signal for spillover calculation using an interval gate. The percentage spillover is calculated based on the median fluorescent intensities of the stained populations and the universal unstained sample. The computed spillover matrix is returned as an R object and written to a named .csv file for future use. spillover_compute has methods for both flowSet and GatingSet objects so refer to their respective help pages for more information.

Author(s)

Dillon Hammill, [e-mail]

References

C. B. Bagwell \& E. G. Adams (1993). Fluorescence spectral overlap compensation for any number of flow cytometry parameters. in: Annals of the New York Academy of Sciences, 677:167-184.

See Also

spillover_compute,flowSet-method

spillover_compute,GatingSet-method

DillonHammill/cytoSuite documentation built on March 7, 2019, 10:09 a.m.