plotCorrStructure: plotCorrStructure

In GabrielHoffman/variancePartition: Quantify and interpret drivers of variation in multilevel gene expression experiments

plotCorrStructure

Description

Plot correlation structure of a gene based on random effects

Usage

plotCorrStructure(
  fit,
  varNames = names(coef(fit)),
  reorder = TRUE,
  pal = colorRampPalette(c("white", "red", "darkred")),
  hclust.method = "complete"
)

Arguments

fit	linear mixed model fit of a gene produced by lmer() or fitVarPartModel()
varNames	variables in the metadata for which the correlation structure should be shown. Variables must be random effects
reorder	how to reorder the rows/columns of the correlation matrix. reorder=FALSE gives no reorder. reorder=TRUE reorders based on hclust. reorder can also be an array of indices to reorder the samples manually
pal	color palette
hclust.method	clustering methods for hclust

Value

Image of correlation structure between each pair of experiments for a single gene

Examples

# load library
# library(variancePartition)

library(BiocParallel)

# load simulated data:
data(varPartData)

# specify formula
form <- ~ Age + (1 | Individual) + (1 | Tissue)

# fit and return linear mixed models for each gene
fitList <- fitVarPartModel(geneExpr[1:10, ], form, info)

# Focus on the first gene
fit <- fitList[[1]]

# plot correlation sturcture based on Individual, reordering samples with hclust
plotCorrStructure(fit, "Individual")

# don't reorder
plotCorrStructure(fit, "Individual", reorder = FALSE)

# plot correlation sturcture based on Tissue, reordering samples with hclust
plotCorrStructure(fit, "Tissue")

# don't reorder
plotCorrStructure(fit, "Tissue", FALSE)

# plot correlation structure based on all random effects
# reorder manually by Tissue and Individual
idx <- order(info$Tissue, info$Individual)
plotCorrStructure(fit, reorder = idx)

# plot correlation structure based on all random effects
# reorder manually by Individual, then Tissue
idx <- order(info$Individual, info$Tissue)
plotCorrStructure(fit, reorder = idx)

GabrielHoffman/variancePartition documentation built on Nov. 8, 2024, 11:17 a.m.