if (interactive()) savehistory();
library("aroma.affymetrix");
log <- Verbose(threshold=-10, timestamp=TRUE);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# WORKAROUNDS
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# The 'GenomeWideSNP_6.cn.na25.annot.csv' [474,602,222 bytes] contains
# a duplicated column header "Fragment Enzyme Type Length Start Stop",
# which needs to be deleted before running this script. The new file
# length is 474,602,181 bytes. /HB 2008-04-25
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Settings
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
naVersion <- "25";
user <- "HB";
datestamp <- "20080424";
chipType <- "GenomeWideSNP_6";
cdfTags <- "Full";
nbrOfEnzymes <- 2;
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Setup required annotation files
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
if (!exists("cdf")) {
cdf <- AffymetrixCdfFile$byChipType(chipType, tags=cdfTags);
rm(csvList);
}
print(cdf);
if (!exists("csvList", mode="list")) {
csvList <- list();
tagsList <- c(
# main=sprintf( ".na%s", naVersion),
cn=sprintf(".cn.na%s", naVersion)
);
for (key in names(tagsList)) {
tags <- tagsList[[key]];
pathname <- AffymetrixNetAffxCsvFile$findByChipType(chipType, tags=tags);
if (isFile(pathname)) {
csv <- AffymetrixNetAffxCsvFile(pathname);
csvList[[key]] <- csv;
rm(csv);
}
rm(tags);
}
}
print(csvList);
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Import UFL from CSV files
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
tags <- sprintf("na%s,%s%s", naVersion, user, datestamp);
ufl <- NULL;
tryCatch({
ufl <- AromaUflFile$byChipType(getChipType(cdf), tags=tags);
}, error = function(ex) {})
if (is.null(ufl)) {
ufl <- AromaUflFile$allocateFromCdf(cdf, tags=tags, nbrOfEnzymes=nbrOfEnzymes);
}
print(ufl);
for (kk in seq_along(csvList)) {
csv <- csvList[[kk]];
print(csv);
units <- importFrom(ufl, csv, verbose=log);
str(units);
}
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# Statistics
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
print(ufl);
x <- summaryOfUnits(ufl, enzymes=c("NspI", "StyI"));
print(x);
## snp cnp affxSnp other total
## enzyme1-only 246080 451191 0 0 697271
## enzyme2-only 160899 0 0 0 160899
## both 522472 494615 0 0 1017087
## missing 2495 20 3022 621 6158
## total 931946 945826 3022 621 1881415
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