rnaNormalize: Normalize a data.table of coverage by RNA seq per position

View source: R/experiment_plots.R

rnaNormalizeR Documentation

Normalize a data.table of coverage by RNA seq per position

Description

Normalizes per position per gene by this function: (reads at position / min(librarysize, 1) * number of genes) / fpkm of that gene's RNA-seq

Usage

rnaNormalize(coverage, df, dfr = NULL, tx, normalizeMode = "position")

Arguments

coverage

a data.table containing at least columns (count/score, position), it is possible to have additionals: (genes, fraction, feature)

df

an ORFik experiment

dfr

an ORFik experiment of RNA-seq to normalize against. Will add RNA normalized to plot name if this is done.

tx

a GRangesList of mrna transcripts

normalizeMode

a character (default: "position"), how to normalize library against rna library. Either on "position", normalize by number of genes, sum of reads and RNA seq, on tx "region" or "feature": same as position but RNA is split into the feature groups to normalize. Useful if you have a list of targets and background genes.

Details

Good way to compare libraries

Value

a data.table of normalized transcripts by RNA.


JokingHero/ORFik documentation built on Dec. 21, 2024, 12:01 a.m.