ex_dcq: Example dCq values data set

Description Usage Format Source

Description

A fictional data set of randomly generated Cq values. Any outliers have been removed with qpcr_clean, using a threshold of 1. Technical replicates have been averaged with qpcr_avg. dCq values have been calculated using qpcr_dcq.

Usage

1

Format

A tibble with 15 rows and 6 variables:

treatment

Three fictional treatment conditions, where CTRL is the negative control

bio_rep

Number to denote which biological replicate the value belongs to

primer_pair

Three fictional genes targeted for qPCR, where gene_hk is the housekeeping gene

cq

Number of PCR cycles need to cross a fluorescence threshold

cq_hk

Cq values of housekeeping control

dcq

Cq value gene of interest - Cq value housekeeping gene

Source

Fictional data set created in excel


JorikBot/tidyqpcr documentation built on Jan. 28, 2021, 11:44 a.m.