ex_ddcq: Example ddCq values data set
In JorikBot/tidyqpcr: Function for tidy ddCt qPCR analysis
Description
Usage
Format
Source
Description
A fictional data set of randomly generated Cq values. Any outliers have been
removed with qpcr_clean, using a threshold of 1. Technical replicates
have been averaged with qpcr_avg. dCq values have been calculated
using qpcr_dcq. ddCq values have been calculated using qpcr_ddcq
Usage
1
Format
A tibble with 10 rows and 9 variables:
- treatment
Three fictional treatment conditions, where CTRL is the
negative control
- bio_rep
Number to denote which biological replicate the value
belongs to
- primer_pair
Three fictional genes targeted for qPCR, where gene_hk
is the housekeeping gene
- cq
Number of PCR cycles need to cross a fluorescence
threshold
- cq_hk
Cq values of housekeeping control
- dcq
Cq value gene of interest - Cq value housekeeping gene
- dcq_ctrl
dCq values of the negative control samples
- ddcq
dCq treated - dCq control
- fold_change
Estimated fold change in gene expression. 2^-ddcq
Source
Fictional data set created in excel
JorikBot/tidyqpcr documentation built on Jan. 28, 2021, 11:44 a.m.
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Description Usage Format Source
Description
A fictional data set of randomly generated Cq values. Any outliers have been
removed with qpcr_clean, using a threshold of 1. Technical replicates
have been averaged with qpcr_avg. dCq values have been calculated
using qpcr_dcq. ddCq values have been calculated using qpcr_ddcq
Usage
1 |
Format
A tibble with 10 rows and 9 variables:
- treatment
Three fictional treatment conditions, where CTRL is the negative control
- bio_rep
Number to denote which biological replicate the value belongs to
- primer_pair
Three fictional genes targeted for qPCR, where gene_hk is the housekeeping gene
- cq
Number of PCR cycles need to cross a fluorescence threshold
- cq_hk
Cq values of housekeeping control
- dcq
Cq value gene of interest - Cq value housekeeping gene
- dcq_ctrl
dCq values of the negative control samples
- ddcq
dCq treated - dCq control
- fold_change
Estimated fold change in gene expression. 2^-ddcq
Source
Fictional data set created in excel
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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