qpcr_dcq: Calculate dCq values
In JorikBot/tidyqpcr: Function for tidy ddCt qPCR analysis

Description Usage Arguments Value Examples

View source: R/qpcr_dcq.R

qpcr_dcq subtracts the housekeeping gene Cq values from the experimental genes Cq values.

1	qpcr_dcq(.data, cq, pcr_target, housekeeping)

`.data`	A data frame or tibble.
`cq`	Unquoted expression. Give the name of the column containing the (averaged) Cq values. If you used the qpcr_avg_techrep function this column is called 'cq'.
`pcr_target`	Unquoted expression. Supply the name of the column that denotes which gene was the target of the qPCR.
`housekeeping`	Quoted expression. Give the value of your housekeeping gene as it occurs in your pcr_target column.

Returns the same type as the input (e.g. a data frame or tibble). Creates 2 new columns. "cq_hk" moves the housekeeping Cq values into its own column. "dcq" contains the calculated dCq values.

dcq_values <- qpcr_dcq(ex_avg,
                       cq = cq,
                       pcr_target = primer_pair,
                       housekeeping = "gene_hk")