downsampleSeurat: Downsample single cell data

In NMikolajewicz/scMiko: scRNAseq analysis functions. Developed using Seurat framework.

Downsample single cell data

Description

Downsample number of cells in Seurat object by specified factor

Usage

downsampleSeurat(
  object,
  subsample.factor = 1,
  subsample.n = NULL,
  sample.group = NULL,
  min.group.size = 500,
  seed = 1023,
  verbose = T
)

Arguments

object	Seurat Object
subsample.factor	Numeric [0,1]. Factor to downsample data by.
subsample.n	Numeric [1,ncol(object)]. Number of cells to subsample. If specified, overides subsample.factor.
sample.group	Character. Meta data grouping variable in which min.group.size will be enforced.
min.group.size	Numeric [1,ncol(object)]. Minimum number of cells to downsample to within sample.group. If there are insufficient cells to achieve the target min.group.size, only the available cells are retained.
seed	seed for sampling. Default is 1023.
verbose	Print progress. Default is TRUE.

Value

Seurat Object

Author(s)

Nicholas Mikolajewicz

NMikolajewicz/scMiko documentation built on June 28, 2023, 1:41 p.m.