plotHeatmap: Heatmap

In acidgenomics/DESeqAnalysis: Acid Genomics DESeq2 Analysis Utilities

Heatmap

Description

Heatmap

Usage

plotCorrelationHeatmap(object, ...)

plotHeatmap(object, ...)

plotQuantileHeatmap(object, ...)

## S4 method for signature 'DESeqAnalysis'
plotHeatmap(object, ...)

## S4 method for signature 'DESeqAnalysis'
plotCorrelationHeatmap(object, ...)

## S4 method for signature 'DESeqAnalysis'
plotQuantileHeatmap(object, ...)

Arguments

object	Object.
...	Additional arguments.

Value

Plot.

Scaling

Here we're scaling simply by calculating the standard score (z-score).

• mu: mean.

• sigma: standard deviation.

• x: raw score (e.g. count matrix).

• z: standard score (z-score).

z = (x - mu) / sigma

See also:

• pheatmap:::scale_rows().

• scale() for additional scaling approaches.

Hierarchical clustering

Row- and column-wise hierarchical clustering is performed when clusterRows and/or clusterCols are set to TRUE. Internally, this calls hclust(), and defaults to the Ward method.

Automatic hierarchical clustering of rows and/or columns can error for some datasets. When this occurs, you'll likely see this error:

Error in hclust(d, method = method) :
NA/NaN/Inf in foreign function call

In this case, either set clusterRows and/or clusterCols to FALSE, or you can attempt to pass an hclust object to these arguments. This is recommended as an alternate approach to be used with pheatmap(), which is called internally by our plotting code. Here's how this can be accomplished:

mat <- assay(mat)
dist <- dist(mat)
hclust <- hclust(dist, method = "ward.D2")

Note

Updated 2021-03-15.

Author(s)

Michael Steinbaugh, Rory Kirchner

See Also

• pheatmap::pheatmap().

• RColorBrewer::brewer.pal().

• stats::cor().

• stats::hclust().

Examples

data(deseq)

## DESeqAnalysis ====
plotHeatmap(deseq)
plotCorrelationHeatmap(deseq)
plotQuantileHeatmap(deseq)

acidgenomics/DESeqAnalysis documentation built on March 27, 2024, 10:32 p.m.