SubsetByBarcodeInflections: Subset a Seurat Object based on the Barcode Distribution...

In atakanekiz/Seurat3.0: Tools for Single Cell Genomics

Description

This convenience function subsets a Seurat object based on calculated inflection points.

Usage

SubsetByBarcodeInflections(object)

Arguments

object	Seurat object
...	arguments to be passed to [CalculateBarcodeInflections()]; if provided, will recalculate the inflection points, else will use those already in 'object'

Details

See [CalculateBarcodeInflections()] to calculate inflection points and [BarcodeInflectionsPlot()] to visualize and test inflection point calculations.

Value

Returns a subsetted Seurat object.

Author(s)

Robert A. Amezquita, [e-mail]

See Also

CalculateBarcodeInflections BarcodeInflectionsPlot

Examples

pbmc_small <- CalculateBarcodeInflections(
  object = pbmc_small,
  group.column = 'groups',
  threshold.low = 20,
  threshold.high = 30
)
SubsetByBarcodeInflections(object = pbmc_small)

atakanekiz/Seurat3.0 documentation built on May 26, 2019, 2:33 a.m.