Description Usage Arguments Value See Also Examples
Give the ChemoProtSet object the correct parameters for a given experiment in order to successfully run the pipelin
1 2 3 4 5 6 7 8 9 10  | setParameters(x, chansVal, repsVal, dataTypeStr, modelTypeStr, PDBool = TRUE,
  removePepsBool = NA, incPDofPDBool = FALSE, PDofPDname = NA,
  incGeneFileBool = FALSE, organismStr = "h.sapiens", sigmoidConc = NA,
  pearsonThrshVal = 0.4)
## S4 method for signature 'ChemoProtSet'
setParameters(x, chansVal, repsVal, dataTypeStr,
  modelTypeStr, PDBool = TRUE, removePepsBool = NA, incPDofPDBool = FALSE,
  PDofPDname = NA, incGeneFileBool = FALSE, organismStr = "h.sapiens",
  sigmoidConc = NA, pearsonThrshVal = 0.4)
 | 
x | 
 object of class 'ChemoProtSet'  | 
chansVal | 
 number of channels / concentrations in experiment  | 
repsVal | 
 number of replicates in experiment  | 
dataTypeStr | 
 string describing the data type of input data set. This can be 'LFC' for log fold-changes, 'FC' for fold-changes and 'intensity' for peptide intensities  | 
modelTypeStr | 
 string describing the type of model applied. This can be 'linear' for a linear model or 'sigmoid' for a sigmoidal model  | 
PDBool | 
 boolean value indicating if the input data is from Proteome Discoverer 2.1 or not  | 
removePepsBool | 
 boolean value indicating if peptide removal will take place. Only valid if input data is peptide intensities  | 
incPDofPDBool | 
 boolean value indicating if the input data contais a pull-down of pull-down colum  | 
PDofPDname | 
 string with the same name as colulmn containing pull-down of pull-down data. NA if this is not applicable  | 
incGeneFileBool | 
 boolean value indicating if the data requires a protein accession to gene ID conversion file  | 
organismStr | 
 string giving the name of organism. the options are: 'H.sapiens', 'D. melanogaster', 'C. elegans', 'R. norvegicus', 'M. musculus'. This is only needed if PDbool is FALSE  | 
sigmoidConc | 
 vector of numerical values for concentrations of channels in the case of a sigmoidal fit  | 
pearsonThrshVal | 
 numerial value between -1 and 1 which determines the cut-off used to discard peptides during peptide removal  | 
object of class ChemoProtSet
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15  | channelNames <- c('Abundance..F1..126..Control..REP_1',
'Abundance..F1..127..Sample..REP_1',  'Abundance..F1..128..Sample..REP_1',
'Abundance..F1..129..Sample..REP_1',  'Abundance..F1..130..Sample..REP_1',
'Abundance..F1..131..Sample..REP_1',  'Abundance..F2..126..Control..REP_2',
'Abundance..F2..127..Sample..REP_2', 'Abundance..F2..128..Sample..REP_2',
'Abundance..F2..129..Sample..REP_2',  'Abundance..F2..130..Sample..REP_2',
'Abundance..F2..131..Sample..REP_2')
ex <- new('ChemoProtSet')
ex<- setParameters(x = ex,chansVal = 6, repsVal = 2,dataTypeStr = 'intensity',
modelTypeStr = 'linear',PDBool = FALSE, removePepsBool = FALSE,
incPDofPDBool = FALSE, incGeneFileBool = FALSE,
organismStr = 'H.sapiens', pearsonThrshVal = 0.4)
ex
 | 
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