get.marker.spillover: Get spillover coefficients

Description Usage Arguments Value References See Also

View source: R/get_marker_spillover.r

Description

Calculates spillover coefficients with robust linear models.

Usage

1
get.marker.spillover(scale.untransformed, flow.gate, flow.control, asp)

Arguments

scale.untransformed

Logical indicating if calculation has to be run in untransformed or transformed (bi-exponential) scale.

flow.gate

List of vectors with ids of gated events per sample.

flow.control

List with data and metadata of a set of controls.

asp

List with AutoSpill parameters.

Value

List of two matrices, with regressions intercepts and coefficients.

References

Roca et al: AutoSpill: A method for calculating spillover coefficients to compensate or unmix high-parameter flow cytometry data. bioRxiv 2020.06.29.177196; doi:10.1101/2020.06.29.177196 (2020).

See Also

gate.flow.data, read.flow.control, and get.autospill.param.


carlosproca/autospill documentation built on Dec. 19, 2021, 1:52 p.m.