read.flow.control: Read set of flow controls

In carlosproca/autospill: AutoSpill: A method for calculating spillover coefficients to compensate or unmix high-parameter flow cytometry data

Description

Read data and metadata from a set of single-color controls.

Usage

read.flow.control(control.dir, control.def.file, asp)

Arguments

control.dir	Character string with the directory with the set of single-color controls.
control.def.file	Character string with the CSV file defining the names and channels of the single-color controls.
asp	List with AutoSpill parameters.

Value

List with the following elements:

• antigen

• autof.marker.idx

• event

• event.n

• event.number.width

• event.sample

• expr.data.max

• expr.data.max.ceil

• expr.data.min

• expr.data.tran

• expr.data.untr

• figure.scatter.dir

• flow.set

• gate.parameter

• marker

• marker.n

• marker.original

• sample

• scatter.and.marker

• scatter.and.marker.label

• scatter.and.marker.original

• scatter.parameter

• transform

• transform.inv

• wavelength

References

Roca et al: AutoSpill: A method for calculating spillover coefficients to compensate or unmix high-parameter flow cytometry data. bioRxiv 2020.06.29.177196; doi:10.1101/2020.06.29.177196 (2020).

See Also

get.autospill.param.

carlosproca/autospill documentation built on Dec. 19, 2021, 1:52 p.m.