process.posnegpop: Process data with positive and negative populations

In carlosproca/autospill: AutoSpill: A method for calculating spillover coefficients to compensate or unmix high-parameter flow cytometry data

Description

Calculates spillover coefficients using positive and negative populations, and the corresponding compensation error.

Usage

process.posnegpop(marker.spillover.unco.untr, flow.gate, flow.control, asp)

Arguments

marker.spillover.unco.untr	List of two matrices, with regressions intercepts and coefficients, resulting from the initial spillover calculation in untransformed scale.
flow.gate	List of vectors with ids of gated events per sample.
flow.control	List with data and metadata of a set of controls.
asp	List with AutoSpill parameters.

Value

List with three elements:

• Calculated spillover matrix.

• Calculated compensation matrix.

• Resulting compensation error, a list with four matrices: intercepts, coefficients, slopes, and skewness.

References

Roca et al: AutoSpill: A method for calculating spillover coefficients to compensate or unmix high-parameter flow cytometry data. bioRxiv 2020.06.29.177196; doi:10.1101/2020.06.29.177196 (2020).

See Also

get.marker.spillover, gate.flow.data, read.flow.control, and get.autospill.param.

carlosproca/autospill documentation built on Dec. 19, 2021, 1:52 p.m.