calculate_concentration: Calculate template concentration

View source: R/concentration.R

calculate_concentrationR Documentation

Calculate template concentration

Description

Calculate template concentration in each wells in a plate using the same formula that QuantaSoft uses. The concentration information is added to the plate's metadata.

Usage

calculate_concentration(plate)

Details

The concentration in a well as number of copies of template per microlitre of sample, and uses the following equation:

(-log(drops_empty / drops_total) * drops_total) / (droplet_volume * drops_total)

Value

A ddPCR plate with the metadata containing a new 'concentration' variable.


daattali/ddpcr documentation built on March 27, 2024, 6:50 a.m.