get_filled_border: Get border of filled droplets in PNPP experiment

In daattali/ddpcr: Analysis and Visualization of Droplet Digital PCR in R and on the Web

Get border of filled droplets in PNPP experiment

Description

In a PNPP experiment, the rain droplets are the non-empty drops that don't have a high enough intensity in the positive dimension to be considered as filled with high quality sample DNA. Only droplets considered as filled are candidates for the negative and positive clusters. get_filled_border returns the threshold value in the positive dimension that is used to determine which drops are filled.

Usage

get_filled_border(plate, well_id)

Arguments

plate	A ddPCR plate.
well_id	Get border of filled droplets for this well.

Value

Thresholds of filled drops in the positive dimension.

See Also

pnpp_experiment
positive_dim
get_filled_drops

Examples

file <- system.file("sample_data", "small", "analyzed_pnpp.rds", package = "ddpcr")
plate <- load_plate(file)
get_filled_border(plate, "A05")
get_filled_border(plate, "F05")

daattali/ddpcr documentation built on March 27, 2024, 6:50 a.m.