PollenGliaData: Obtain the Pollen radial glia data

Description Usage Arguments Details Value Author(s) References Examples

View source: R/PollenGliaData.R

Description

Obtain the human radial glia single-cell RNA-seq dataset from Pollen et al. (2017).

Usage

1
PollenGliaData(ensembl = FALSE, location = TRUE)

Arguments

ensembl

Logical scalar indicating whether the output row names should contain Ensembl identifiers.

location

Logical scalar indicating whether genomic coordinates should be returned.

Details

Column metadata includes the anatomical source, sample of origin, presumed cell type and assorted alignment statistics.

If ensembl=TRUE, the gene symbols are converted to Ensembl IDs in the row names of the output object. Rows with missing Ensembl IDs are discarded, and only the first occurrence of duplicated IDs is retained.

If location=TRUE, the coordinates of the Ensembl gene models are stored in the rowRanges of the output. This is only performed when ensembl=TRUE.

All data are downloaded from ExperimentHub and cached for local re-use. Specific resources can be retrieved by searching for scRNAseq/pollen-glia.

Value

A SingleCellExperiment object with a single matrix of read counts.

Author(s)

Aaron Lun

References

Pollen A et al. (2017). Molecular identity of human outer radial glia during cortical development. Cell 163, 55-67.

Examples

1
sce <- PollenGliaData()

drisso/scRNAseq documentation built on Feb. 16, 2021, 1:18 a.m.