ERCCSpikeInConcentrations: Obtain ERCC concentrations
In drisso/scRNAseq: Collection of Public Single-Cell RNA-Seq Datasets

Description Usage Arguments Details Value Author(s) Examples

View source: R/ERCCSpikeInConcentrations.R

Obtain ERCC spike-in concentrations from the Thermo Fisher Scientific website.

1	ERCCSpikeInConcentrations(volume = NULL, dilution = NULL, mix = c("1", "2"))

`volume`	Numeric scalar specifying the added volume (in nanoliters) of ERCC spike-in mixture. Only used if `dilution` is specified.
`dilution`	Numeric scalar specifying the dilution factor used for the added volume of the spike-in mixture. Only used if `volume` is specified.
`mix`	String specifying whether to compute the number of molecules for mix 1 or 2. Only used if both `dilution` and `volume` are specified.

If volume and dilution are specified, an additional column is added to the output specifying the number of molecules of spike-in transcipt for the specified mix.

All data are downloaded from ExperimentHub and cached for local re-use. Specific resources can be retrieved by searching for scRNAseq/ercc-concentrations.

A DataFrame object with one row per ERCC spike-in transcript. This contains information such as the spike-in concentration in each mix.

Alan O'Callaghan