debarcoderPrepareFcs: Prepare FCS data for debarcoding.

View source: R/debarcoder.R

debarcoderPrepareFcsR Documentation

Prepare FCS data for debarcoding.

Description

Transform expression data using cofactor, scale to [0..1] range, match to barcoding channels, and sort each event separately.

Usage

debarcoderPrepareFcs(fcs, key, cofactor = 10)

Arguments

fcs

FCS flow frame.

key

Barcoding key data frame.

cofactor

Before scaling, data will be transformed using inverse hyperbolic sin with given cofactor.

Value

Expression data after transformation, scaling, and matching, and an additional copy after sorting.


ismmshimc/cytutils documentation built on Aug. 26, 2022, 2:07 p.m.