H1N1_Flow: longitudinal cell population frequencies and titer response...

In juliancandia/eNetXplorer: Quantitative Exploration of Elastic Net Families for Generalized Linear Models

longitudinal cell population frequencies and titer response upon H1N1 vaccination

Description

Data from a cohort of healthy subjects vaccinated against influenza virus H1N1. Cell population frequencies from deep-phenotyping flow cytometry were determined longitudinally pre- (days -7, 0) and post-vaccination (days 1, 7, 70). The response is the adjusted maximum fold change (adjMFC) of serum titers at day 70 relative to baseline, as defined in Tsang et al.

Usage

data(H1N1_Flow)

Format

For each timepoint (days -7, 0, 1, 7, 70), a numerical matrix of predictors is provided with subjects as rows and cell populations as columns. Two versions of the serum titer response are given: response_numer as a numerical vector and response_class as a categorical vector discretized into low (0), intermediate (1) and high (2) response classes. A metadata file with cell population annotations is also provided.

Details

Cell populations were manually gated and expressed as percent of parent. Samples and cell populations were filtered independently for each timepoint. Samples filter: excluded if median of viable cells fraction across all 5 tubes was <0.7. Cell population filter: excluded if >80% of samples had <20 cells. Data adjustment: data were log10-transformed and pooled across all timepoints, then adjusted for age, gender and ethnicity effects. For more details, see Tsang et al.

References

Tsang JS et al. Global Analyses of Human Immune Variation Reveal Baseline Predictors of Postvaccination Responses, Cell (2014) 157: 499-513.

juliancandia/eNetXplorer documentation built on May 11, 2023, 1:59 a.m.