split_big_flowFrames: Split big flow frames into smaller fcs files

In prybakowska/CytoQP: Cytometry data quality control and cleaninig

Split big flow frames into smaller fcs files

Description

Split the big flow frames into smaller ones.

Usage

split_big_flowFrames(
  flow_frame,
  event_per_flow_frame = 5e+05,
  out_dir = NULL,
  min_cell_per_fcs = 20000
)

Arguments

flow_frame	Flow frame containing cytometry data.
event_per_flow_frame	Numeric, the number of events to be split to small flow frames, default is set to 500000.
out_dir	Character, pathway to where the files should be saved, default is set to file.path(getwd(), Splitted).
min_cell_per_fcs	Numeric, minimal number of cells in flow frame to save fcs file, default 20000.

Value

Save splitted fcs files in out_dir.

Examples

ff <- flowCore::read.FCS(list.files(path = raw_data_dir,
pattern = ".FCS", full.names = TRUE)[1])

split_big_flowFrames(flow_frame = ff, event_per_flow_frame = 100000)

prybakowska/CytoQP documentation built on June 28, 2022, 12:36 a.m.