docs/README.md

In rahuldhodapkar/melange: Multi-Modal Data Import Utilities for Seurat

melange

There are many tools available to generate data for single cell analytics, and as the field matures, the need to join these data together increases. melange is a toolkit intended to provide intuitive and efficient data transformation and manipulation utilities for use with the popular Seurat R package.

Currently Supporting

• RSEM transcript quantitation by gene

• Annovar variant annotation hits by gene

• MiXCR B- and T- cell receptor clonotype analysis by cell

Installation Instructions

To install a development release of melange, simply clone this repository

git clone [e-mail]:rahuldhodapkar/melange.git

and use devtools utilities. For version 3.5.3 of R, the invocation is:

devtools::install("path/to/melange")

At this point, melange will be available for use as a normal R package in your environment by library(melange) when required.

To simplify the installation process, you can also navigate to the cloned melange directory and use:

make install

which will run the above installation.

Developing for melange

If you are working with output of a research script and have developed an in-house solution for data integration with your single-cell sequencing workflow, please contact us (rahul.dhodapkar at yale.edu). We would love to include your work as part of melange so others may benefit from your efforts!

rahuldhodapkar/melange documentation built on Nov. 5, 2019, 2:07 a.m.