pipe.GenomicAlign | R Documentation |
Runs the genomic alignment step to place reads from FASTQ files onto genomic chromosomal locations.
pipe.GenomicAlign(inputFastqFile, sampleID,
optionsFile = "Options.txt", asMatePairs = FALSE, verbose = TRUE,
rawReadCount = NULL)
inputFastqFile |
character vector of one or more raw FASTQ files |
sampleID |
the SampleID for this sample. This SampleID keys for a row of annotation details in the annotation file, for getting sample-specific details. The SampleID is also used as a sample-specific prefix for all files created during the processing of this sample. |
optionsFile |
the file of processing options, which specifies all processing
parameters that are not sample specific. See |
asMatePairs |
logical flag, should the vector of FASTQ be treated as two mate pair files? |
rawReadCount |
optional argument of the number of reads in the files. By default this gets calculated on the fly. |
Aligns the given FASTQ files against the Bowtie2 genomic index specified in the options file. Reads
that do align are written to a BAM file in the align
results subfolder. Reads that fail to align are written
to temporary file(s) in the current working folder for the optional subsequent splice alignment step.
a family of BAM files, FASTQ files, and summary files, written to subfolders under the results.path
folder.
Also, a list of alignment counts:
RawReads |
the number of reads in the raw FASTQ files |
UniqueReads |
the number of reads that aligned to exactly one location |
MultiReads |
the number of reads that aligned to two or more locations |
NoHitReads |
the number of reads that failed to align |
Time |
the time usage of this alignment step, as from |
Bob Morrison
pipe.RiboClear
Ribo clearing alignment against an index of unwanted genomic featurs.
pipe.SpliceAlign
Splice junction alignment against an index of standard and alternative splice junctions.
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